Comparative in vitro activity of medicinal plants arnebia densiflora and ecballium elaterium against isolated strains of klebsiella pneumoniae

İzole klebsiella pneumoniae suşlarma karşı tıbbi bitkilerden arnebia densiflora ve ecballium elaterium un karşılaştırmah in vitro aktivitesi

Özet

Ecballium elaterium ve Arnebia densiflora (Nordm.) ledeb, ekstreleri, disk difüzyon testinde (trimetoprim-sulfametoksazol, sulbaktam-ampisilin, klavulonat-amoksisilin, seftriakson, sefepim, imipenem, seftazidim, tobramisin, gentamisin, oflokzasin, siproflokzasin) direngli izole suş Klebsiella pneumoniae’e karsi tarandı. Duyarhhk testi olarak Klinik ve laboratuvar Standartlan Enstitüsii önerileri dogrultusunda sıvı mikrodilüsyon testi uygulandı. A. densfilora 'rim kök kabuğu ve E. elaterium yaprak, govde ve meyva ekstreleri izole K pneumoniae suşuna ve kontrole (ampisilin and ofloksasin) karsi 128 ve 0 0312 pLg/ml araliginda değişen etki gösterdi. Bu galisma A. densiflora ve E. elaterium ekstrelerinin izole K pneumoniae (K2, K3, K5, K& ve K10) suşlarma karsi 32 /ug/ml konsantrasyonunda amoksisilin ve ofloksasin antibiyotikleri kadar etkili olduğunu gösteren ilk rapordur. Ekstreler, geri kalan suşlarda 64 jug/ml konsantrasyonunda aktiftirler, bu da kontrolün etkili dozuna yahndır. Bununla birlikte turn ekstreler standart kontrol suş K pneumoniae (RSKK 574) ’ye karsi (8 jUg/I) izole suş K pneumoniae ’ye 32 ve 64 jug/ml arasında değişen etkiden karsilaştırmah olarak daha iyidir.

Abstract

In vitro activity of the extracts of medicinal plants Ecballium elaterium and Arnebia densiflora, which is endemic to Turkey, was screened against isolated strains of Klebsiella pneumoniae that were resistant in disc diffusion test (trimethoprime-sulfamethoxazol, sulbactam-ampicillin, clavulonat-amoxicillin, ceftriaxone, cefepime, imipenem, ceftazidime, tobramycin, gentamicin, ofloxacin, ciprofloxacin). Broth microdilution susceptibility testing was performed according to the Clinical and laboratory Standards Institute. The extracts of A. densfilora root bark and E. elaterium leaf stem and fruit samples were analyzed against the strains of K. pneumoniae at the concentrations range from 128 to 0.0312 pLg/ml. This is the first report shows that the extracts of A. densiflora and E. elaterium are effective as much as the antibiotics amoxicillin and ofloxacin against some of the isolated strains of K. pneumoniae (K2, K3, K5, K& and K10) at the concentration of 32 jug/mL. The extracts were active at the concentration of 64 jug/mL onto rest of the strains, which are close to the effective dose of controls as well. However, the activities of all the extracts against standard control strain of K. pneumoniae (RSKK 574) were comparatively better (8 jug/ml) than that of the tested isolated strains of K. pneumoniae, in which the activities varied between 32 to 64 jug/ml.