Lipid peroxidation and apoptosis levels in gastric tissue of spontaneously hypertensive rats

Spontan hipertansif syçanlaryn mide mukozasynda lipid peroksidasyon ve apopitozis düzeyleri

Özet

Amaç: Spontan hipertansif syçanlar esansiyel hipertansiyon için yaygyn olarak kullanylan bir hayvan modelidir.Hipertansiyonun mide üzerindeki, gastrite ba?ly kanama, mide mukozal kan akymy ve motilite de?i?iklikleriile damar geni?lemesi gibi çe?itli komplikasyonlarynyn bilinmesine kar?yn, ?imdiye kadar hipertansifhayvanlaryn mide mukozal apopitozisiyle ilgili ara?tyrma yapylmamy?tyr. Bu çaly?mada, spontan hipertansifsyçanlaryn midelerinde lipid peroksidasyon düzeyleri ve apopitotik de?i?iklikler ara?tyryldy. Gereç ve Yöntem:Spontan hipertansif (n:10) ve normotansif Wistar Kyoto (n:10) ratlar kullanyldy. Mide mukozasynda apopitozisintespiti için terminal deoxynucleotdyl transferase-mediated deoxyuridine triphosphate nick-end labellingtekni?i, lipid peroksidasyonunun göstergesi olarak thiobarbituric acid reactive substances düzeyleri ölçüldü.Bulgular: Spontan hipertansif syçanlarda apopitotik hücre sayysynda Wistar Kyoto syçanlarla kar?yla?tyryldy?yndaanlamly bir arty? saptanyrken thiobarbituric acid reactive substances düzeyleri de?i?memi?tir. Sonuç: Sonuçolarak, bu çaly?ma ile ilk kez hipertansif syçanlaryn mide mukozasynda apopitozisde bir arty? gösterilmi?tirancak apopitozise yol açan mekanizmalar kesin de?ildir

Abstract

Lipid peroxidation and apoptosis levels in gastric tissue of spontaneously hypertensive ratsAim: The spontaneously hypertensive rat is a widely used animal model for essential hypertension . Despitethe recognized gastric complications of hypertension, such as bleeding from gastritis, gastric mucosal bloodflow and motility and vascular ectasias until now no investigations have been conducted on gastric mucosalapoptosis in hypertensive animals. In this study, we investigated the lipid peroxidation and apoptoticchanges in the stomachs on spontaneously hypertensive rats. Methods: Spontaneously hypertensive (n: 10)and normotensive Wistar Kyoto rats (n: 10) were used. Thiobarbituric acid reactive substances weredetermined as an indicator of lipid peroxidation and terminal deoxynucleotdyl transferase-mediateddeoxyuridine triphosphate nick-end labelling technique was used to detect apoptosis in gastric tissue. Results:While the number of apoptotic cells were increased significantly in spontaneously hypertensive rats,thiobarbituric acid reactive substances were not changed when compared Wistar Kyoto rats. In conclusion,data presented in the current study demonstrate the significant increase in gastric mucosal apoptosis inspontaneously hypertensive rats for the first time but underlying mechanisms of apoptosis are not clarified.