UrinemiR-21-5pandmiR-200c-3paspotential non-invasivebiomarkersin patientswithprostatecancer

Özet

Objective: To evaluate the miR-21-5p and miR-200c-3p expressions in the urine of patients with prostatecancer (PCa) and to investigate their potential as biomarkers.Material and methods: The urine samples collected from 80 patients, including 20 patients diagnosed withbenign prostate hyperplasia (BPH) and 60 patients diagnosed with PCa, were examined. The exosome isolation was performed using the miRCURY exosome isolation kit (Exiqon, Denmark), total RNA was extractedusing the miRCURY RNA Isolation Kit-Biofluid kit (Exiqon, Denmark), and complementary DNA (cDNA)was synthesized using the Universal cDNA Synthesis kit (Exiqon, Denmark). A quantitative polymerasechain reaction (qPCR) analysis of gene expression was performed using the qPCR CFX 96 Thermocycler(Bio-Rad). All the procedures followed the manufacturer’s recommendations.Results: The overexpressions of miR-21 in the non-metastatic PCa and metastatic PCa groupcompared to the BPH group were statistically significant with a p-value of 0.001 and 0.018, respectively. The non-metastatic PCa compared to the metastatic PCa group was also statisticallysignificant with a p-value of 0.037. The under expressions of miR-200c in the non-metastatic PCaand metastatic PCa group compared to the BPH group are statistically significant with a p-valueof 0.001 and 0.001, respectively.Conclusion: The overexpressions of miR-21 found in this study could be a potential non-invasive diagnostictool for patients with PCa. Despite the significant results in our study, the use of micro-RNA in urine samples may vary due to epigenetic variation. Further studies with larger populations are required to investigatethe role of miR-21 and miR-200c as biomarkers in PCa.