Characterization of Phosphate Solubilising Bacteria from Limestone Quarry in Cirebon Indonesia

Abstract

Limestone quarry has low available P for plant on land revegetation process. Therefore, phosphate-solubilising bacteria could be a solution to dissolve phosphate in limestone in order to add phosphate availability. The research was aimed to characterize and identify the phosphate-solubilising bacteria adapted in limestone quarry. Isolation was performed using Pikovskaya medium which obtained four isolates bacteria had halo-zone around the colony. The ability to dissolve phosphate was examined using five soil samples from different region of limestone quarry. The result showed index of P solubility between 1.63 to 3.42. Identification bacterial isolates by using 16S rRNA gene showed that the isolates have similarity with Pseudomonas mosselii QC5A.1, Pseudomonas mosselii QC5B.1, Pantoea ananatis QC5C.1, and Pseudomonas oryzihabitans QC5B.3.