The effect of combined resveratrol and nitric oxide treatment on the prevention of spinal cord ischemia-reperfusion injury: An experimental study

Özet

Objectives: The aim of this study was to investigate protective effects of resveratrol (an antioxidant) alone and in combination withLG-nitro-L-arginine-methyl ester (L-NAME), a nitric oxide synthase [NOS] inhibitor, against spinal cord ischemia-reperfusion injury inan experimental rabbit model.Materials and methods: Twenty-four rabbits were used in the study. The study was conducted on 24 rabbits assigned into four groups asfollows: Group 1, sham group; Group 2, control group; Group 3, resveratrol group; and Group 4, resveratrol + L-NAME group. The bloodsamples were drawn before abdominal exposure and at 48 h. Resveratrol 10 mg/kg and L-NAME 3 mg/kg were given to the test subjects.The animals were sacrificed after neurological assessment at 48 h, and spinal cord tissues were removed for histopathological examination.Results: No significant difference was observed in biochemical analyses, while there were significant differences in neurological andhistopathological examination results among the groups. The highest functional neurological improvement (as assessed by Tarlov score:4.5±0.57) was observed in the resveratrol + L-NAME group (p<0.05). A significant improvement was recorded in the resveratrol +L-NAME group (3.2±0.5) in histopathological examination (p<0.05).Conclusion: In the experimental ischemia-reperfusion model used in this study, it was proven that resveratrol + L-NAME combination hadmore favorable histopathological and neurological effects on spinal cord injury, compared to resveratrol alone.