Dergiler / KSÜ Tarım ve Doğa Dergisi / 2020 / Cilt: 23 - Sayı: 1
Determination of Phytochemical Profile of Allium tuncelianum and Evaluation of Its Antiproliferative Effect on Various Human Cell Lines
- Sayfa
- 259–270
- DOI
- —
Özet
In this study, Allium tuncelianum, one of the endemic garlic speciesgrowing in Anatolia, was investigated for its phytochemical content.LC-MS/MS, HPLC, GC-MS, GC-FID and ICP-OES techniques wereused for this purpose. Major phenolic components of Alliumtuncelianum were found to be malic acid (3322.6 µg/g), kainic acid(626.8 µg/g), cinnamic acid (69.15 µg/g), fumaric acid (13.02 µg/g) andcatechin (3933.3 µg/g). The main components of volatile oil, on theother hand, were detected as diallyl disulfide (28.30%), diallyltrisulfide (30.90%) and allyl methyl trisulfide (9.44%). The fatty acidcomposition of the plant was revealed as oleic acid (27.19%), linoleicacid (19.46%) and elaidic acid (3%) whereas high content of potassium(4207± 67 mg/kg), calcium (518± 35 mg/kg) and magnesium (376 ± 20mg/kg) was determined as the minerals. Moreover, cytotoxic effects ofethanol/water and hexane/chloroform extracts of the plant wereevaluated in prostate, colon, cervical and breast cancer cell lines andcytotoxicity of both extracts were detected for all the cell lines studied.Therefore, we conclude that Allium tuncelianum may be a newphytotherapy agent with its rich phytochemical content andanticancer activity.
Abstract
Bu çalışmada, Anadoluda yetişen endemik sarımsak türlerinden biri olan Tunceli Dağ Sarımsağı (Allium tuncelianum) incelenmiştir. Fitokimyasal içeriği belirlemek için LC-MS / MS, HPLC, GC-MS, GCFID ve ICP-OES teknikleri kullanılmıştır. Allium tuncelianum'un başlıca fenolik bileşenleri; malik asit (3322,6 µg / g), kainik asit (626.8 µg / g), sinamik asit (69.15 µg / g), fumarik asit (13.02 µg / g) ve kateşin (3933.3 µg / g) olduğu belirlendi. Uçucu yağın ana bileşenleri, dialil disülfit (% 28.30), dialil trisülfit (% 30.90) ve allil metil trisülfit (% 9.44) idi. Yağ asidi bileşimi; oleik asit (% 27.19), linoleik asit (% 19.46) ve elaidik asit (% 3) iken, mineral içeriğindeki major elementler; potasyum (4207 ± 67 mg / kg), kalsiyum (518 ± 35 mg / kg) ve magnezyum (376 ± 20) mg / kg) olarak belirlenmiştir. Prostat, kolon, servikal ve meme kanseri hücre dizilerinde sitotoksik etki de incelenmiştir. Kolon, Meme, Servikal ve Prostat kanseri hücre hatlarında sitotoksik etki tespit edildi. Bu sonuçlar göstermektedir ki; Allium tuncelianum, zengin fitokimyasal içeriği ve antikanser aktivitesi ile yeni bir fitoterapi ajanı olabilir.