Dergiler / Türk Üroloji Dergisi/Turkish Journal of Urology / 2004 / Cilt: 30 - Sayı: 2
Asetilkolinin in vitro veziküla seminalis kontraksiyonlarına etkisi
- Sayfa
- 143–147
- DOI
- —
Özet
Veziküla seminalisler gerek sekresyonları ve gerekse de kontraktil özellikleri ile erkek fertilitesinde önemli rol oynayan organlardır. Hem sempatik hem de parasempatik sinir sistemi tarafından inerve edilen veziküla seminalislerin, ayrıca pürinerjik uyaranlarla da inerve edildikleri belirlenmiştir. Bu çalışmada da deneysel olarak asetilkolinin veziküla seminalis kas kontraksiyonlarına etkinliğinin araştırılması amaçlandı.Çalışmada 200-250 g ağırlığında, erişkin erkek Wistar sıçanlardan izole edilen veziküla seminalis kesitleri kullanıldı. Hayvanlar dekapite edildikten sonra veziküla seminalisleri alındı ve 0.5x0.2 cm boyutlarında kesitler hazırlandı. Hazırlanan kesitler, içerisinde %95 oksijen ve %5 karbondioksitle sürekli gazlandırılan 37oC’de Krebs solüsyonu bulunan ısı ceketli çift çeperli izole organ banyosunda 1 gramlık istirahat gerimi altında asılarak izometrik kasılmaları kayıt edildi. Asetil kolin (10 µM Ach) organ banyosuna uygulanarak kasılmalar indüklendi. İlave olarak atropin uygulanarak asetilkolinle indüklenen kontraksiyonlar üzerine etkileri incelendi. Kasılmaların amplitütleri miligram (mg) olarak, frekansları ise kasılma sayısı (kasılma sayısı/ 10dakika) olarak hesaplandı. Asetilkolinin konsantrasyona bağımlı bir şekilde izole sıçan seminal vezikül kesitlerinde kasılmalara yol açtı. $10^{-9}$, $10^{-6}$ ve $10^{-5}$ M’lık ACh ile indüklenen kasılmaların maksimum değerleri sırasıyla 0.7±1.4 mg, 985±145 mg ve 1189±156 mg idi. Atropin uygulaması ACh ile indüklenen kasılma yanıtlarını hemen başlayan bir etki ile tamamen inhibe etti. Asetilkolin, veziküla seminalislerde atropinle inhibe edilebilen aktif kontraksiyonlara sebep olmaktadır. Bu kontraksiyonların çeşitli spesifik droglarla manüplasyonu gerek erkek infertilitesinde, gerekse prematüre ejakülasyon tedavisinde araştırılması gereken yeni konulardır.
Abstract
Introduction: Seminal vesicles play important roles in male fertility by both their secretions and contractile characteristics. With the intense muscle mass in their wall and contractile features of these muscles, they are not only involved in fertility but also in ejaculation period. Since involved in ejaculation, regulations of tonus of this muscle will erve a new approach to the premature ejaculation that is among to the most common male sexual dysfunction. Determination of involvement of purinergic system in the innervation of these vesicles which is known to have sympathetic as well as parasympathetic innervations has lead to the investigation of mechanisms involved in the regulation of this organ. In the present experimental study, it was aimed to determine the effects of acethylcholine (ACh) on the contractility of isolated seminal vesicle. Materials and Methods: Seminal vesicle strips used in this study were isolated from 200-250 g male Wistar Following decapitation, the lower abdomen was opened and seminal vesicles were removed and about 0.5 x 0.2 cm strips were obtained. The strips were placed in were placed in a jacketed organ bath containing Krebs’ solution at 370C and pH 7.4, constantly gassed with 95% O2-5% C02 and after equilibration under one gram of resting tension isometric contractions were recorded. After equilibration under 1 gram of resting tension, a 10-minute control recording was performed from strips those developed spontaneous contractions. At the end of this period ACh (10 µM) was applied to the organ bath to induce contractions and then atropine was applied to determine its effect on these ACh-induced contractions. Amplitude (mg) and frequency (number of contractions/10-min) of contractions and area under the contraction curves were evaluated by 10-minute intervals. Results: Spontaneous contractions, even small, were developed in strips studied during the equilibration period. Maximal contractile response to the acethylcholine $10^{-9}$, $10^{-6}$ and $10^{-5}$ M were recorded. The mean maximal contractile response to ACh ($10^{-9}$, $10^{-6}$ and $10^{-5}$ M) were 0.7±1.4 mg, 985±145 mg and 1189±156 mg, respectively. Atropine was inhibited the ACh-induced contractile responses by an immediately started effect. Atropine (1 micro molar) inhibited the area under the contractile curve induced by 10-5 M ACh by 85%; which was reached to a complete inhibition within 220±35 s of atropine application. Conclusion: In rat seminal vesicle ACh causes contractions, which are, can be inhibited by atropine. Investigation of the effects of variety of specific agents on these contractions is of interest with respect to the development of therapy for male infertility and premature ejaculation.