Dergiler / Selçuk Tıp Dergisi / 2001 / Cilt: 17 - Sayı: 2
Yüksek doz A vitamininin karaciğer üzerine etkileri ışık mikroskobik çalışma
- Dergi
- Selçuk Tıp Dergisi
- Sayfa
- 73–77
- DOI
- —
Özet
Bu çalismada yüksek doz all-trans retinoik asidin karaciger dokusu üzerine olan etkileri arastirildi. Çalismada 12 adet ergin erkek Wistar cinsi siçan kullanildi. Denekler 3 gruba ayrildi. I.grup: On bes gün boyunca oral yoldan 20 mg/kg/gün all-trans retinoik asit verilen siçanlar (n:4) II.grup: On bes gün boyunca oral yoldan 40 mg/kg/gün all-trans retinoik asit verilen siçanlar (n:4) III.grup: Kontrol olarak kullanilan siçanlardan olusturuldu. (n:4) Deneyin sonunda siçanlar eter anestezisi altinda öldürülüp karaciger dokulari alinip isik mikroskobik incelemeler için Boin solüsyonunda tespit edildi. Parafin bloklar hazirlanip 5 mm'lik kesitler alindi. Hazirlanan kesitlere farkli boyalar uygulanip isik mikroskopta incelendi. Deney gruplarinin karaciger lobüllerinin periferal zonundaki hepatosit sitoplazmalarinin boyanmadigi görüldü. Yine lobüllerin periferal zonlarinda aktive olmus Kupffer hücre yogunlasmalari görüldü. Bu bulgularin paralelinde deney gruplarinin lobül yapilarinda bozulmalar saptandi. Yapisal degisiklikler 40mg/kg/gün all-trans retinoik asit verilen gruplarda daha siddetli düzeyde idi.
Abstract
The effects of excess all-trans retinoic acid on liver tissue were investigated in this study. Twelve adult male Wistar rats were used. The animal were divided into three groups. I. group: 20 mg/kg/day all-trans retinoic acid was given to rats (n:4) by oral gavage, during 15 days II.group: 40 mg/kg/day all-trans retinoic acid was given to rats (n:4) by oral gavage, during 15 days III.group: Rats were used as control (n:4) End of experimental study, rats were killed under ether anesthesia. Liver tissues were taken and fixed with Boin's solution for light microscobic observation. Paraffin blocks were prepared and 5 mm sections were stained diffrent methods. Later, sections were examined with ligth microscope. In the experimental groups, the cytoplasms of hepatocytes in the peripheral zone of liver lobules were stainless. Excess cell infiltration which were considered activated Kupffer cells, observed at the peripheral zone. Moreover, hepatic lobule structure of experimental groups were destroyed. This findings were more serious for intake 40 mg/kg/day all-trans retinoic acid rats.