Dergiler / EurAsian Journal of BioSciences (elektronik) / 2009 / Sayı: 3
The effect of sensitivity to abscisic acid on stomatal behaviour in Arabidopsis thaliana
- Sayı
- 2009 · Sayı: 3
- Sayfa
- 10–16
- DOI
- —
Özet
Stoma osilasyounu, asiri kuraklik kosullari altinda su kaybinin onlenmesi, terleme oraninin azaltilmasi ve su kullaniminin etkinliginin artirilmasinda etkin bir mekanizma olarak tanimlanmistir. Onceki arastiricilar; ABA'nin tetikledigi H2O2 uretimi ve H2O2'nin harekete gecirdigi Ca2+ kanallarinin, ABA'ca tetiklenen stoma salinimi icin onemli bir mekanizma oldugunu gostermislerdir. Bu calismada, absisik asit (ABA) duyarliliginin stoma osilasyonunu nasil duzenledigi, Arabidopsis thaliana mutantlari (abi1-1, ABA'ya duyarsiz ve era1-2, ABA'ya asiri duyarli) ve yabani tipler kullanilarak arastirilmistir. Sonuclar, (1) ABA'ya asiri duyarliligin ABA'ca tetiklenen stoma osilasyonunu ve kapanmasini guclendirdigini, (2) ABA'ya duyarsizligin, ABA'ca tetiklenen stoma osilasyounu ve kapanmayi ortadan kaldirdigini ve (3) stoma osilasyonu, kapanma ve absisik asite duyarlilik arasinda positif bir iliski oldugunu gostermistir. Veriler, ABA'ya duyarliligin Arabidopsis thaliana'da stoma osilasyonu ve kapanmayi duzenliyor olabilecegini gostermektedir.
Abstract
Stomatal oscillation has been described as an efficient mechanism to prevent water loss, decrease the transpiration rate and to improve water use efficiency under severe drought conditions. Former researches demonstrated that ABA-induced H2O2 production and H2O2- activated Ca2+ channels were important mechanism for ABA-induced stomatal oscillation. In this study, how sensitivity to abscisic acid (ABA) regulates stomatal oscillation was investigated with Arabidopsis thaliana mutants (abi1-1, insensitivity to ABA and era1-2, hypersensitive to ABA) and wild types. The results showed that (1) hypersensitivity to ABA strengthens stomatal oscillation and closure induced by ABA; (2) insensitivity to ABA abolishes stomatal oscillation and closure induced by ABA; and (3) there is a positive relationship between stomatal oscillation, closure and sensitivity to abscisic acid. The data indicates that sensitivity to ABA may regulate stomatal oscillation and closure in Arabidopsis thaliana.