Dergiler / EurAsian Journal of BioSciences (elektronik) / 2010 / Sayı: 4
Effects of 24-epibrassinolide on growth, chlorophyll, electrolyte leakage and proline by pepper plants under NaCl-stress
- Sayı
- 2010 · Sayı: 4
- Sayfa
- 96–104
- DOI
- —
Özet
Brassinosteroidler, çevresel streslerin üstesinden gelme özelligine sahip steroid yapisinda bitki hormonlaridir. Bu çalisma, 24-epibrassinolit'in biber bitkisinde tuz toleransi tetiklemedeki rolünü incelemek üzere gerçeklestirilmis ve su fizyolojik parametreler ölçülmüstur: Büyüme, klorofil, elektrolit kaybi ve prolin. Capsicum annuum cv. Beldi tohumlari üzerine, NaCl varliginda ve yoklugunda 24-epibrassinolit püskürtüldü ve uygulamadan 28 gün sonra örnek alindi. Tuzluluk stresi altindaki kültürlerin analizi sonucu, biyokütle ve klorofilin önemli ölçüde azaldigi, elektrolit kaybi ve prolin yogunlugunun ise tuzluluk stresi altinda önemli miktarda arttigi tespit edildi. Ancak, 24-epibrassinolit uygulamasi, incelenen parametrelerde tuzluluk stresinin olumsuz etkilerini önemli ölçüde hafifletti ve bu durum, harici 24-epibrassinolit uygulamasinin stress altindaki bitkilerde büyümeyi artirabilecegi ve hücre zarinin yapisini koruyabilecegini iddia eden önceki yazarlarin varsayimlarini dogrular niteliktedir.
Abstract
Brassinosteroids are steroidal phytohormones that have the ability to overcome plant environmental stress. This study was carried out to investigate the role of 24-epibrassinolide in inducing pepper plant salt tolerance as measured by a range of physiological parameters: growth, chlorophyll, electrolyte leakage and proline. Capsicum annuum cv. Beldi seedlings were sprayed with 24-epibrassinolide both in the presence or the absence of NaCl and were sampled, 28 days after treatments. As a result of analysing the cultures under salinity stress, it was determined that the biomass and the chlorophyll decreased significantly, while the electrolyte leakage and the proline concentration increased considerably under salinity stress. However, the application of 24-epibrassinolide significantly ameliorated the adverse effects of salinity on the examined parameters, confirming the suppositions of previous authors who have claimed that exogenously applied 24-epibrassinolide can increase growth and protect the integrity of the cellular membrane in stressed plants.