Dergiler / EurAsian Journal of BioSciences (elektronik) / 2010 / Sayı: 4
Coumarins from Zosima absinthifolia seeds, with allelopatic effects
- Sayı
- 2010 · Sayı: 4
- Sayfa
- 17–22
- DOI
- —
Özet
Apiaceae üyesi olan Zosima absinthifolia, Iran, Türkiye, Irak ile Kafkaslar, Orta Dogu ve Orta Asya'nin degisik ülkelerinde bulunur. Meyveleri gida tatlandiricisi olarak ve Iran'da baharat olarak kullanilir. Bu calismada; bitki tohumunun n-hekzan özütleri, imperatorin isimli bir furanokumarin ile 7-preniloksi kumarin ve orapten isimli iyi bilinen 2 kumarin elde etmek icin, vakum sivi kromatografi ve preparatif TLC ile saflastirildi. Bilesik yapilari UV, 1H ve 13C NMR verileri araciligi ile elde edildi. Bulgularimiz, tüm bitki bilesiklerinin, özellikle de imperatorinin, yaygin bir patojen olan Sclerotinia sclerotorium'a karsi fungal toksik aktivitesi oldugunu gösterdi. Bilesikler ayrica fitotoksik etki sergilediler ve tohum cimlenmesini, filiz ve kök gelisimini engellediler. Buradan, saflastirilan bilesiklerin bitkiler icin allelopatik rol oynadiklari ve patojenlerle rakip otlara karsi bitkiyi koruyabilecegi sonucuna varilabilir.
Abstract
Zosima absinthifolia belongs to the Apiaceae family and is found in Iran, Turkey, Iraq and different countries of the Caucasus, Middle East and Central Asia. The fruits are used as food flavoring and as a food spice in Iran. In the present work, an n-hexane extract of the plant seeds was purified by vacuum liquid chromatography and preparative TLC for affording a furanocoumarin named imperatorin and two known coumarins, 7-prenyloxy coumarin and aurapten. The compound structures were elucidated by UV, 1H and 13C NMR data. Our results indicated that all tree compounds, especially imperatorin exhibited fungi toxic activity against Sclerotinia sclerotiorum, a common plant pathogen. The compounds also displayed phytotoxic effects and stunted seed germination, shoot and root growth of lettuce. It could be concluded that the purified compounds play allelopathic roles for the plant and could protect the plant against pathogens and competing herbs.