Evaluation of oxidative stress factors in patients with osteoporosis

Abstract

Osteoporosis is an important disease with an increasing incidence that leads to serious losses in physical function and adversely affecting the life quality of women especially in postmenopausal period. Age, genetic structure, vitamin D and K abnormalities, estrogen deficiency, chronic inflammation and oxidative stress are considered among the most important risk factors for osteoporosis. Therefore, we aimed to investigate the plasma paraoxonase (PON1) activity, highdensity lipoprotein (HDL), total sialic acid (TSA) and total oxidant/antioxidant status (TOS/TAS) of patients with osteoporosis in this study. Comparisons of 25 female patients diagnosed with osteoporosis aged between 51-67 and 10 healthy women between the ages ranged 50-68 were made in this study. PON1 activity, HDL, TSA, TOS and TAS levels were measured by spectrophotometrical method in plasma samples that obtained from blood samples of patients and healthy individuals. Results were expressed as mean ± standard deviation. Plasma PON1 activity, TAS and HDL levels were significantly lower in patiens with osteoporosis than the healty group (p<0.001, p<0.001 and p<0.01 respectively). Also, plasma TSA and TOS levels were higher in patients with osteoporosis compared to the healthy group were found statistically significant (p<0.01). Increased reactive oxygen species in patients with osteoporosis may play an important role by causing oxidative stres in the pathogenesis of osteoporosis. Based on oxidative stres, increasing levels of oxidant and reducted levels of antioxidant molecules indicates that may be an important indicator of bone loss in women. Also, higher plasma sialic acid levels in the patients with osteoporosis may be caused by increasing in of sialic acid secretion from the cell membrane surface that based on oxidative tissue damage