Dergiler / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2018 / Cilt: 8 - Sayı: 2
Koruyucu Madde Paraben’in Allium cepa L.’daki Fizyolojik, Anatomik ve Sitogenetik Etkilerinin Araştırılması
- Sayfa
- 65–71
- DOI
- —
Özet
Bu çalışmada, günlük yaşamımızda sıkça kullandığımız kozmetik ve temizlik ürünleri başta olmak üzere,
Abstract
In this study, toxicity induced by Allium cepa L. test material of Methyl Paraben which is included inmany products, especially cosmetic and cleaning products which we use frequently in our daily life, was investigatedby using physiological, cytogenetic and anatomical parameters. Physiological parameters; germination percentage,root length and weight gain, cytogenetic parameters; by determining the micronucleus frequency, chromosomalabnormalities and mitotic index, anatomical parameters were investigated by microscopic examination of thesections taken from the root tips. A. cepa L. seeds were divided into four (4) groups, one (1) control and three(3) applications, for 72 hours, the seeds in the control group were treated with tap water and the seeds in thetreatment group were treated with doses of 100, 250 and 500 mM of Methyl Paraben. At the end of the period,root tips were subjected to routine preparations, stained and made ready for microscopic examinations. Eventually,there was a statistically significant decrease (p<0.05) in the germination percentage, root length, weight gain andmitotic index values of the Paraben application, although there was a statistically significant an increase (p<0.05)in the micronucleus and chromosomal abnormality numbers. In addition, Paraben administration has resulted inanatomical changes in the root cells of the flattened cell nucleus, necrosis, thickening of the cell walls of the cortex,nonspecific vascular tissue, cell deformation, and accumulation of certain substances in the cortex cells. As a result,it has been shown that Paraben shows toxic effect at a certain dose level and A. cepa L. test material is a usefulbiological indicator in determining its effect