Dergiler / Gülhane Tıp Dergisi / 2000 / Cilt: 42 - Sayı: 3
In-vitro initial cytotoxicity of luting agents
- Dergi
- Gülhane Tıp Dergisi
- Sayfa
- 231–234
- DOI
- —
Özet
Yapıştırma ajanları karıştırıldıktan sonra sitotoksisite potansiyeli olan içerik salınımları yapmaktadırlar. Bu çalışmada üç resin simanın (Permaflex:P, Optibond O, Metabond C&B:M), iki polikarboksilat simanın (Shofu Hy-Bond Polycarboxylate cement.SP, Express Polycarboylate cement:XP) ve iki cam iyonomer simanın (Meron Glass lonomer cementMG, Shofu Hy-Bond Glass ionomer cement:SG) başlangıç sitotoksik etkileri He-La hücre kültüründe incelenmiştir. Materyal örnekleri karıştırıldıktan sonra steril silikon kalıplar içerisine yerleştirilerek Eagle's minimum essential medium ve feutal calf serum (EMEM+FCS) içerisine daldırılarak elde edilen test materyali, monolayer hücre ve EMEM+FCS içeren viallere inoküle edilmiş ve sito¬toksik etki hücrelerde oluşan morfolojik de?işikilikler 24 saatlik periodlarla incelenerek belirlenmiştir. 0,P,Mve SP örnekleri diğerleine göre daha fazla sito¬toksik etki göstermişlerdir.
Abstract
Luting agents release potentially cytotoxic ingredi¬ents during setting. In this study, the initial cytotoxic effects of three resin luting cements (Permaflex.P, Optibond:0, Metabond C&B:M), two Zinc phosphate cements (Shofu Zn Phosphate Cement :SZ, Express Zinc Phosphate Cement: XZ), two Polycarboxylatecements (Shofu Polycarboxylate Cement.SP, Express Polycarboxylate Cement: XP), and two glass ionomer cements (VOCO Meron Glass lonomer Luting Cement: MG, Shofu Glas lonomer Luting Cement: SG) on He-La cell culture were investigated. Material specimens prepared in presterilized silicone standard jigs according to manufacturers directions and immediately immersed in 1ml (EMEM). Cells were reproduced in flasks containing % 10 Feutal calf serum (FCS) and Eagle's minimum essential medium (EMEM) as to form a monolayer. Then removed, cen-trifugated, and diluted to 3.106 cell/ml in EMEM+FCS solution and placed in 96-wells microplate for incuba¬tion in 37°C02 for two hours.0.05 ml of test material was inoculated in eight wells for each material and 0.05 ml EMEM-FCS was inoculated in eight wells as controls. The morphological changes in cells were observed in 24 hour periods with a cell culture micro¬scope. 0, P, M and SP specimens were more toxic than MG, SG, XZ, XP and SZ specimens. All speci¬mens showed cytotoxic effects at 48 hours.