Dergiler / Journal of the Entomological Research Society / 2015 / Cilt: 17 - Sayı: 2
Molecular Identification of Western-Palaearctic Leaf Beetles (Coleoptera, Chrysomelidae)
- Sayfa
- 93–101
- DOI
- —
Özet
ABSTRACT Due to the difficulties associated with morphological identification of insects, it became necessary to resort to other identification tools, such as DNA barcoding, where the mitochondrial cytochrome c oxidase subunit I (COI) molecular marker is commonly used. The effectiveness of DNA-based identification of species relies on the availability of sequences in public databases for comparison. Nevertheless, there is still a large number of non-sequenced species in these databases, preventing a molecular identification. In this study, we generate COI barcode sequences, with a total of 658 bp, for the six studied Chrysomelidae species. Phylogenetic and sequence divergence analyses were also performed, which allowed the discrimination of all species under study, supporting once again the suitability of this genetic marker. The obtained sequences were added to BOLD and GenBank databases, contributing to the increase of records in online databases and making the identification of some Chrysomelidae species easier. Key words: Coleoptera, Chrysomelidae, DNA barcoding, Cytochrome oxidase I, databases.
Abstract
ABSTRACT Due to the difficulties associated with morphological identification of insects, it became necessary to resort to other identification tools, such as DNA barcoding, where the mitochondrial cytochrome c oxidase subunit I (COI) molecular marker is commonly used. The effectiveness of DNA-based identification of species relies on the availability of sequences in public databases for comparison. Nevertheless, there is still a large number of non-sequenced species in these databases, preventing a molecular identification. In this study, we generate COI barcode sequences, with a total of 658 bp, for the six studied Chrysomelidae species. Phylogenetic and sequence divergence analyses were also performed, which allowed the discrimination of all species under study, supporting once again the suitability of this genetic marker. The obtained sequences were added to BOLD and GenBank databases, contributing to the increase of records in online databases and making the identification of some Chrysomelidae species easier. Key words: Coleoptera, Chrysomelidae, DNA barcoding, Cytochrome oxidase I, databases.