Restore a 9 mm diameter osteochondral defect with gene enhanced tissue engineering followed mosaicplasty in a goat model

Abstract

Objective: The aim of this study was to evaluate the efŞcacy of gene enhanced tissue engineering followed mosaicplasty in a goat model.Methods: An acute cylindrical defect 9 mm in diameter was created in the weight bearing area of themedial femoral condyle in a goat model. Thirty-six medial femoral condyles were divided into 6 groupsusing different proportion of gene enhanced tissue engineering and mosaicplasty to restore the defects.The specimen received gross and histology observation, which was evaluated by the histological gradingscale of O'Driscoll, Keeley and Salter. Transmission electron microscope observation was also performed.Two factors analysis of variance and Student-Newman-Kewls test were used to compare the specimen.Results: The gross and histology observation revealed that each defects of six groups had differentrestoration. The scores of the reparative tissue of three groups with gene enhancement were signiŞcantlyhigher than those in other three groups without gene enhancement (p> 0.05).Conclusion: Gene enhanced tissue engineering followed mosaicplasty could restore a 9 mm diameterosteochondral defects in a goat model effectively. With the reduction of covering area of the graft, theadvantages of the combined gene enhanced tissue engineering method can be better reflected.© 2016 Turkish Association of Orthopaedics and Traumatology. Publishing services by Elsevier B.V. This isan open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)