The Relationship Between Plasma Adipsin, Adiponectin, Vaspin, Visfatin, and Leptin Levels with Glucose Metabolism and Diabetes Parameters

Özet

Introduction: Recently, interesting adipokines have been described that affect glucose metabolism and insulin resistance. However, the relationships between these adipokines and their importance in the pathogenesis of diabetes are contradictory. The aim of the present study was to investigate the relationship between plasma adipsin, adiponectin, vaspin, visfatin, and leptin levels in diabetic rats with liver enzymes related to glucose metabolism and diabetes parameters. Methods: Wistar albino rats were divided into three groups: control, diabetic control, and treatment groups, each with 7 of them. One group of diabetic rats was orally treated with 200 mg/kg/day metformin, and the other diabetic group received placebo. After 6 weeks, the animals were sacrificed by cardiac puncture. Glucose, insulin, glycosylated hemoglobin (HbA1c), adipsin, adiponectin, visfatin, vaspin, and leptin levels in blood were measured by enzyme-linked immunosorbent assay (ELISA), and insulin resistance was determined. In liver homogenates, glucose-6-phosphate dehydrogenase (G6PD), glucose- 6-phosphatase (G6P-ase), glucokinase, pyruvate kinase (PK), and fructose-1.6-diphosphatase (F1.6DP) activities were measured by ELISA. Results: Dietary food intake and water consumption, fasting blood glucose and HbA1c levels, and insulin resistance of diabetic rats were higher than those of the control and treatment groups. Plasma adipsin, adiponectin, vaspin, and leptin levels significantly decreased, and visfatin level increased in diabetic rats. G6PD, glucokinase, and PK activities decreased, whereas G6P-ase and F1.6DP activities increased in diabetic rats. There were a negative correlation between plasma adipsin and adiponectin levels and insulin resistance and a positive correlation between visfatin level and insulin resistance in diabetic rats. In addition, adipsin, adiponectin, and leptin levels were positively correlated with each other and with some liver enzymes. Discussion and Conclusion: In the diabetic rat model, body weight, glucose metabolism enzymes, insulin sensitivity, and changes in plasma adipsin, adiponectin, vaspin, visfatin, and leptin levels indicate that these adipokines play an important role in the glucose metabolism, insulin resistance, and pathogenesis of diabetes.