Assessment of Elemental Content, Antioxidant Activity, and Total Phenolic Content of Vitis sylvestris Gmelin

Abstract

In this study, we determined the Cu, Fe, Mn, and Zn contents of Vitis sylvestris Gmelinusing flame atomic absorption spectrometry (FAAS), and examined the antioxidant properties of Vitissylvestris Gmelin using radical scavenging capacities and total phenolic content tests. We found theaverage elemental contents; Cu, Fe, Mn, and Zn as 1.506 ± 0.042 mg/kg, 0.796 ± 0.020 mg/kg,2.333 ± 0.033 mg/kg, and 3.191 ± 0.262 mg/kg, respectively. When we examined the antioxidantactivity tests applied to different extracts, we determined the highest extraction yield with themethanol extract. DPPH radical scavenging activity, ABTS radical scavenging activity, and totalphenolic content values in methanol extract were determined to be respectively 3.957 ± 0.146 mgTEAC/g fw, 9.062 ± 0.273 mg TEAC/g fw, and 2.365 ± 0.028 mg GAE/g fw. When we evaluated theantioxidant activity and total phenolic content results for all extracts statistically, we determined thatthere was generally a statistically significant difference between each extract (p < 0.01). Vitissylvestris Gmelin has high antioxidant content when considering the data obtained. Also, wedetermined that it is an effective candidate in the protection against reactive oxygen species.