Dergiler / Turkish Journal of Chemistry / 2015 / Cilt: 39 - Sayı: 3
Characterization of a Cu$^{2+}$-selective fluorescent probe derived from rhodamine B with 1,2,4-triazole as subunit and its application in cell imaging
- Sayfa
- 660–666
- DOI
- —
Özet
A rhodamine B derivative containing 1,2,4-triazole as subunit was characterized as an "off-on" type Cu$^{2+}$-selective fluorescent probe. It exhibited high selectivity and sensitivity for Cu$^{2+}$ in ethanol-water solution (9:1, v:v, pH 7.0, 20 mM HEPES) and underwent ring opening. A prominent fluorescence enhancement at 570 nm was observed in the presence of Cu$^{2+}$ with the change in the absorption spectrum, and a 1:1 metal-ligand complex was formed. With the optimized experimental conditions, the probe exhibited a dynamic response range for Cu$^{2+}$ from 8.0 $\times $ 10$^{-7}$ to 7.5 $\times $ 10$^{-6}$ M with a detection limit of 2.3 $\times $ 10$^{-7}$ M in ethanol-water solution (9:1, v:v, pH 7.0, 20 mM HEPES). Its application in Cu$^{2+}$ imaging in living cells was also studied.
Abstract
A rhodamine B derivative containing 1,2,4-triazole as subunit was characterized as an “off–on” type Cu 2+ - selective fluorescent probe. It exhibited high selectivity and sensitivity for Cu 2+ in ethanol–water solution (9:1, v:v, pH 7.0, 20 mM HEPES) and underwent ring opening. A prominent fluorescence enhancement at 570 nm was observed in the presence of Cu 2+ with the change in the absorption spectrum, and a 1:1 metal–ligand complex was formed. With the optimized experimental conditions, the probe exhibited a dynamic response range for Cu 2+ from 8.0 × 10 −7 to 7.5 × 10 −6 M with a detection limit of 2.3 × 10 −7 M in ethanol–water solution (9:1, v:v, pH 7.0, 20 mM HEPES). Its application in Cu 2+ imaging in living cells was also studied.