Febuxostat Mitigates Genotoxicity Induced by Ionizing Radiation in Human Normal Lymphocytes

Özet

Febuxostat (BF) is a xanthine oxidase inhibitor that has been used to treat chronic gout. FB exhibits an anti-inflammatory effect. In the present study, the radioprotective effect of BF was investigated against genotoxicity induced by ionizing radiation (IR) in healthy human lymphocytes. Peripheral blood samples were treated with BF at various concentrations for 3 h. Then, the whole blood samples were exposed to IR at a dose of 1.5 Gy. Lymphocytes were cultured to determine the frequency of micronuclei in binucleated lymphocytes. The frequency of micronuclei was significantly lower in human lymphocytes that were exposed to IR and treated with BF than in irradiated lymphocytes without BF treatment. The maximum radioprotection as decreasing in the frequency of micronuclei was observed at 100 μM of BF treatment (43% decrease). The anti-inflammatory property is probably involved in the mechanism of FB exerting radioprotective effect in human lymphocytes.