Kırıkkale (Türkiye)’de İnsan, Koyun ve Sığır Topluluklarında Bruselloz Seroprevalansı

Seroprevalence of Brucellosis in Human, Sheep, and Cattle Populations in Kırıkkale (Turkey)

Özet

Türkiye’de K›r›kkale’nin k›rsal kesimlerinde insanlarda, koyun ve s›¤›r topluluklar›nda Brusella serorpevalans› araflt›r›ld›. Serum örnekleri 1436 kifliden elde edildi (% 28,41 erkek ve % 71,59 bayan). Toplumun % 3,2 (46)’si Rose Bengal Plate testi (RBPT) ile ve % 3,0 (43)’ü standard tüp aglutinasyon testi (STAT) ile pozitif olarak sonuç verdi. RBPT ile pozitif olarak bulunan serumlar ELISA testi kullan›larak % 75 (33 kifli) Brucella ELISA IgG ile ve % 25 (11 kifli) Brucella ELISA IgM ile pozitif olarak sonuç verdi. RBPT ile 301 s›¤›rdan ve 503 koyundan olmak üzere toplam 804 serum örne¤inin araflt›r›lmas› sonucu % 47 (52) seropozitiflik gözlendi. RBPT ile pozitif olarak tespit edilen tüm hayvanlar STAT ile de pozitif olarak sonuç verdi. 301 s›¤›r ve 503 koyunda do¤rulama testi olarak kullan›lan Kompleman Fiksasyon Testi (CFT) ile seropozitiflik s›ras›yla % 1 ve % 8 olarak bulundu.

Abstract

The seroprevalence of Brucellosis in human, sheep, and cattle populations was investigated in rural regions of Kırıkkale in Turkey. Serum samples were obtained from 1436 humans, and 3.2% (46) of the population was found to be positive by Rose Bengal Plate test (RBPT) and 3.0% (43) gave positive results with the standard tube agglutination test (STAT). Immunoglobulin class analysis of RBPT-positive sera using the ELISA test gave positive results for 33 people (75%) with Brucella ELISA IgG and for 11 people (25%) with Brucella ELISA IgM. The evaluation of 804 serum samples, which were obtained from 301 cattle and 503 sheep, showed 6.47% (52) seropositivity with RBPT. Additionally, all animals were found to be positive with STAT. The seropositivity was 1% and 8 %, respectively, in serum samples obtained from 301 cattle and 503 sheep with the complement fixation test (CFT), which was used as the confirmatory test.