Dergiler / Etlik Veteriner Mikrobiyoloji Dergisi / 1995 / Cilt: 8 - Sayı: 1
Sığırlarda Paratüberkülozun Serolojik, Allerjik ve Bakteriyolojik Muayene Yöntemleri ile Mukayeseli Olarak Araştırılması
- Sayfa
- 19–44
- DOI
- —
Özet
Paratüberküloz son zamanlarda önem kazanan bir yetiştirme hastalığı olarak ruminantlarda görülmektedir. Bu hastalıktan ileri gelen ekonomik kayıplar önemlidir. Dünyada yaygın olan bu hastalığa ülkemizde de rastlanmaktadır. Çalışmamızda paratüberkülozun çeşitli teşhis yöntemleri ile araştırılması beş ayrı işletmede serolojik, allerjik ve gaita örneklerinin bakteriyolojik yoklaması ile yapıldı ve aşağıdaki sonuçlar alındı. İşletmelerden temin edilen 5118 kan serumu komplement fikzasyon testi ile işlenip 85 pozitif 30 şüpheli reaksiyon alındı. Allerjik teşhisde PPD avian ve PPD johnin kullanılarak 4923 sığırda yapılan tüberkülin testinde 73 pozitif 19 şüpheli reaksiyon tesbit edildi. Serolojik ve allerjik testlerde reaksiyon alınan 145 sığıra PPD johnin uygulandı ve 52 pozitif , 6 şüpheli reaksiyon alındı. Bu sığılardan temin edilen gaita kültürlerinden 15 adedinde M.paratuberculosis izole edilmiştir; ayrıca rektal mukoza kazıntı örneklerinin mikroskopik yoklamalarında 4 pozitif olgu değerlendirilmişitir.
Abstract
Paratüberculosis has recently become an important breed ing disease in ruminants in many countries since it has caused great economic losses. Being worldwide, paratuberculosis alsa occurs in our country . in this study, the use of different diagnostic methods in paratuberculosis was investigated in 5 different farms by serological and allergical tests as well as bacteriological examination of feces samples. The results obtained are as follows : 5118 blood sera from 5 herds were tested with the antigen prepared with M.paratuberculosis 3 and 5 path strains by complement fixation test and 85 positive and 30 suspected sera were found . The sera that responded to paratuberculosis were tested with avian antigen by the complement fixation test and 76 positive and 18 suspected sera were found .Tuberculin (PPD bovine, PPD avian) administration in 4923 catle resulted in 73 positives and 19 suspected to PPD avian and no posit ives to PPD bovine. According to serological and suspected cattle were kept separately. Two months afterwards, PPD Johnin was administered on them . PPD John in tuberculin administered on 145 cattle resulted in 52 pozitives and 6 suspected . 15 M. paratuberculosis isolations were made from rectal mucosa and feces samples from 145 cattle that responded to allerg ical and serological test in bacteriological examinations (Herold egg . yolk medum with and without mycobactin) Furthermore, 9 M. pheli isolations were made, causing transient sensibilisation . In one management, 21 cattle that reacted to PPD avian were negative for PPD Johnin and serological tests . Bacteriological examinations offeces samples from these animals gave negative results. These examinations were carried out three times with 6 month intervals. In bacteriological carried out on starlings that are widely present in winter. Runyon group AAR mycobacteria were isolated. This mycobacterium induce no lesions in laboratory animals. Tuberculin (PPD bovine. PPD avian and PPD Johnin were inoculated into guinea pigs in equal doses and positive reaction occured to PPD avian only. After tuberculin administration two months later on cattle, positive reaction to PPD avian was observed to have disappeared. Ina herd , cattle vaccinated against paratuberculosis responded to PPD avian and Johnin , however, serological tests and PPD bovine results were negative. Ina herd of 22b0 cattle that hadproblemsin the past with paratuberculosis allergical tests were carried out (PPD avian and PPD John in were adm inistered with 6 month interval). In 1990, periodical hygienic applications were made in this management and 34 cattle positive for paratuberculosis in 1990 and 16 positive cattle in the first s ix months of 1991 and 11 positive cattle in the second six months of 1991 were slaughtered . Allergical and serological tests carried out in 1992 and 1993 in complete herd resulted in negative reactions . lt was concluded that this result was a consequent of the change from range system to feedlot system . In the fa rm s on which our'study was carried out no animals with prominent symptoms of paratuberculosis were observed with an exception that the animals positive after feces cultures exhibited repeated diarrhea and there was cachexia in some animals . In the positive animals, infection could be defined as subclinical or latent infection of 15 cattle whose feces cultures were positive , 11 were positive , that is diagnostic efficiency of cultures was 73,2 % i however this was 92,3 % in PPD Johnin .