KaydedilenlerDerginizi ekleyin

Dergiler / Gazi Üniversitesi Mühendislik Mimarlık Fakültesi Dergisi / 2026 / Cilt: 41 Sayı: 2

Aronya ekstraktı içeren Kolajen/HA kompozit kemik greftinin üretimi ve karakterizasyonu

Production and characterization of Aronia extract containing Collagen/HA composite bone graft

Gökçe Kaynak Bayrak

Sayfa
1239–1248
Yayın Tarihi
2026-07-20
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗

Özet

Travma, kazalar veya onkolojik rezeksiyonlar sonucu ortaya çıkan kemik doku kayıpları, etkili doku replasmanı için gelişmiş kemik greft ikamelerinin geliştirilmesini gerekli kılmaktadır. Biyouyumluluk ve osteogenezi destekleme gibi temel gerekliliklerin yanı sıra, rezeksiyon bölgesinde kalabilecek mikroskobik tümör hücrelerini baskılayabilecek antikanserojen fonksiyonelliğin kazandırılması, özellikle tümör sonrası rekonstrüksiyonda önemli bir terapötik üstünlük sağlayacaktır. Aronia melanocarpa, güçlü antioksidan, anti-inflamatuvar, antiproliferatif, antimikrobiyal ve antikanser aktiviteleriyle tanınan zengin biyoaktif fitokimyasal içeriğe sahip bir meyvedir. Bu çalışmada, ilk kez aronya ekstraktı kolajen–hidroksiapatit (Kol/HA) esaslı kompozit kemik greftlerinin üretiminde kullanılarak, yeni ve çok fonksiyonlu bir biyomalzeme platformu ortaya konmuştur. Greftler liyofilizasyon tekniğiyle üretilmiş ve genipin ile çapraz bağlanmıştır. Morfolojik, fizikokimyasal ve yapısal özellikleri; taramalı elektron mikroskobu (SEM), X-ışını kırınımı (XRD), ATR-FTIR ve şişme analizleri kullanılarak kapsamlı biçimde karakterize edilmiştir. Ayrıca, L929 fibroblast hücreleri ile gerçekleştirilen MTT analizleri, geliştirilen greftlerin sitotoksik olmadığını ve biyouyumlu yapıda olduğunu ortaya koymuştur.

Abstract

Bone tissue loss resulting from trauma, accidents, or oncological resections necessitates the development of advanced bone graft substitutes for effective tissue replacement. In addition to fundamental requirements such as biocompatibility and the ability to promote osteogenesis, the incorporation of anticarcinogenic functionality capable of suppressing residual microscopic tumor cells within the resection site would provide a substantial therapeutic advantage, particularly in post-tumor reconstruction. Aronia melanocarpa is a rich source of bioactive phytochemicals and has been widely recognized for its potent antioxidant, anti-inflammatory, antiproliferative, antimicrobial, and anticancer activities. In this study, for the first time, Aronia extract was incorporated into the fabrication of collagen–hydroxyapatite (Col/HA)-based composite bone grafts, introducing a novel multifunctional biomaterial platform. The grafts were fabricated via a lyophilization technique and cross-linked with genipin. Their morphological, physicochemical, and structural properties were comprehensively characterized using scanning electron microscopy (SEM), X-ray diffraction (XRD), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), and swelling analyses. Furthermore, cytocompatibility was confirmed by MTT assays using L929 fibroblast cells, demonstrating the non-cytotoxic nature of the developed grafts.

Anahtar kelimeler
🔖

Bu Makaleye Atıf Yap

Bayrak, G. K. (2026). Aronya ekstraktı içeren Kolajen/HA kompozit kemik greftinin üretimi ve karakterizasyonu. Gazi Üniversitesi Mühendislik Mimarlık Fakültesi Dergisi, Cilt: 41 Sayı: 2, 1239–1248. https://doi.org/10.17341/gazimmfd.1848993

📄 Tam Metin PDF Okuyucu

↗Yeni Sekme

PDF belgesi yükleniyor...

Kaynakça (50)

  1. 1. Feng X., Chemical and biochemical basis of cell-bone matrix interaction in health and disease, Curr. Chem. Biol., 3 (2), 189–196, 2009.
  2. 2. Kumar P., Saini M., Dehiya B.S., Sindhu A., Kumar V., Kumar R., Lamberti L., Pruncu C.I., Thakur R., Comprehensive survey on nanobiomaterials for bone tissue engineering applications, Nanomaterials, 10 (10), 1–28, 2020.
  3. 3. Ahlawat S., Fayad L.M., Revisiting the WHO classification system of bone tumours: emphasis on advanced magnetic resonance imaging sequences, Pol. J. Radiol., 85, e409–e419, 2020.
  4. 4. Zhang D., Wu X., Chen J., Lin K., The development of collagen-based composite scaffolds for bone regeneration, Bioact. Mater., 3 (1), 129–138, 2018.
  5. 5. Kołodziejska B., Kaflak A., Kolmas J., Biologically inspired collagen/apatite composite biomaterials for potential use in bone tissue regeneration, Materials, 13, 1748, 2020.
  6. 6. Hussein M.O., Abdulhameed A.S., Design of bionanomaterial of chitosan carbohydrate polymer composited with broccoli extract and zinc oxide nanoparticles: anticancer activity in human osteosarcoma, Appl. Biochem. Biotechnol., 197, 1073–1089, 2025.
  7. 7. Yurtsever M.Ç., Cömertpay A., Iyigundogdu Z., Room temperature biosynthesis of ZnO nanoparticles using avocado seed extract with antimicrobial and anticancer properties, Int. J. Environ. Sci. Technol., 21, 8025–8038, 2024.
  8. 8. Adamiak K., Sionkowska A., Current methods of collagen cross-linking, Int. J. Biol. Macromol., 161, 550–560, 2020.
  9. 9. Dong C., Lv Y., Application of collagen scaffold in tissue engineering: recent advances and new perspectives, Polymers, 8 (2), 42, 2016.
  10. 10. Rico-Llanos G.A., Borrego-González S., Moncayo-Donoso M., Becerra J., Visser R., Collagen type I biomaterials as scaffolds for bone tissue engineering, Polymers, 13 (4), 599, 2021.
  11. 11. Antoniac I.V., Antoniac A., Vasile E., Tecu C., Fosca M., Yankova V.G., Rau J.V., In vitro characterization of novel nanostructured collagen-hydroxyapatite composite scaffolds doped with magnesium with improved biodegradation rate for hard tissue regeneration, Bioact. Mater., 6 (10), 3383–3395, 2021.
  12. 12. Cunniffe G.M., Dickson G.R., Partap S., Stanton K.T., O’Brien F.J., Development and characterisation of a collagen nano-hydroxyapatite composite scaffold for bone tissue engineering, J. Mater. Sci.: Mater. Med., 21 (8), 2293–2298, 2010.
  13. 13. Karakeçili A., Korpayev S., Orhan K., Optimizing chitosan/collagen type I/nanohydroxyapatite cross-linked porous scaffolds for bone tissue engineering, Appl. Biochem. Biotechnol., 194 (9), 3843–3859, 2022.
  14. 14. Popa C.L., Albu M., Bartha C., Costescu A., Luculescu C., Trusca R., Antohe S., Structural characterization and optical properties of hydroxyapatite/collagen matrix, Rom. Rep. Phys., 68 (3), 1149–1158, 2016.
  15. 15. Bacakova L, Novotna K, Hadraba D, Musilkova J, Slepicka P, Beran M. Influence of biomimetically mineralized collagen scaffolds on bone cell proliferation and immune activation, Polymers (Basel), 14 (3), 602, 2022.
  16. 16. Sotome S., Ae K., Okawa A., Ishizuki M., Morioka H., Matsumoto S., Nakamura T., Abe S., Beppu Y., Shinomiya K., Efficacy and safety of porous hydroxyapatite/type 1 collagen composite implantation for bone regeneration: a randomized controlled study, J. Orthop. Sci., 21 (3), 373–380, 2016.
  17. 17. Borowska S., Brzóska M.M., Chokeberries (Aronia melanocarpa) and their products as a possible means for the prevention and treatment of noncommunicable diseases and unfavorable health effects due to exposure to xenobiotics, Compr. Rev. Food Sci. Food Saf., 15 (6), 982–1017, 2016.
  18. 18. Cebova M., Klimentova J., Janega P., Pechanova O., Effect of bioactive compound of Aronia melanocarpa on cardiovascular system in experimental hypertension, Oxid. Med. Cell. Longev., 2017.
  19. 19. Jurikova T., Mlcek J., Skrovankova S., Sumczynski D., Sochor J., Hlavacova I., Snopek L., Orsavova J., Fruits of black chokeberry Aronia melanocarpa in the prevention of chronic diseases, Molecules, 22 (6), 944, 2017.
  20. 20. Buda V., Brezoiu A.M., Berger D., Pavel I.Z., Muntean D., Minda D., Dehelean C.A., Soica C., Diaconeasa Z., Folescu R., Danciu C., Biological evaluation of black chokeberry extract free and embedded in two mesoporous silica-type matrices, Pharmaceutics, 12 (9), 838, 2020.
  21. 21. Thani N.A.A., Keshavarz S., Lwaleed B.A., Cooper A.J., Rooprai H.K., Cytotoxicity of gemcitabine enhanced by polyphenolics from Aronia melanocarpa in pancreatic cancer cell line AsPC-1, J. Clin. Pathol., 67 (11), 949–954, 2014.
  22. 22. Gao N., Wang Y., Jiao X., Chou S., Li E., Li B., Preparative purification of polyphenols from Aronia melanocarpa with cellular antioxidant and antiproliferative activity, Molecules, 23 (1), 139, 2018.
  23. 23. Gajic D., Saksida T., Koprivica I., Vujicic M., Despotovic S., Savikin K., Jankovic T., Stojanovic I., Chokeberry (Aronia melanocarpa) fruit extract modulates immune response in vivo and in vitro, J. Funct. Foods, 66, 103836, 2020.
  24. 24. Savikin K., Zdunić G., Janković T., Gođevac D., Stanojković T., Pljevljakušić D., Berry fruit teas: Phenolic composition and cytotoxic activity, Food Res. Int., 62, 677–683, 2014.
  25. 25. Sainova I., Pavlova V., Alexieva B., Valkova I., Nikolova E., Experimental models of cell in vitro-differentiation by different laboratory incubation times in the presence of Aronia melanocarpa fruit extract, Int. J. Adv. Res. Biol. Sci., 2 (3), 206–210, 2015.
  26. 26. Woo H.W., Lee J.S., Characterization of electrospun Aronia melanocarpa fruit extracts loaded polyurethane nanoweb, Fashion Text., 8 (1), 1–14, 2021.
  27. 27. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J., Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193, 265–275, 1951.
  28. 28. Xiao F., Xu T., Lu B., Liu R., Guidelines for antioxidant assays for food components, Food Front., 1, 60–69, 2020. 29. Li Z., Du T., Ruan C., Niu X., Bioinspired mineralized collagen scaffolds for bone tissue engineering, Bioact. Mater., 6, 1491–1511, 2021.
  29. 30. Thi N.D., Hwang E.S., Effects of drying methods on contents of bioactive compounds and antioxidant activities of black chokeberries (Aronia melanocarpa), Food Sci. Biotechnol., 25 (1), 55–61, 2016.
  30. 31. Sidor A., Gramza-Michałowska A., Black chokeberry Aronia melanocarpa L.: A qualitative composition, phenolic profile and antioxidant potential, Molecules, 24, 3710, 2019.
  31. 32. Romani A., Vignolini P., Ieri F., Heimler D., Polyphenols and volatile compounds in commercial chokeberry (Aronia melanocarpa) products, Nat. Prod. Commun., 11 (1), 99–102, 2016.
  32. 33. Pieszka M., Gogol P., Pietras M., Valuable components of dried pomaces of chokeberry, black currant, strawberry, apple and carrot as a source of natural antioxidants and nutraceuticals in the animal diet, Ann. Anim. Sci., 15 (2), 475–491, 2015.
  33. 34. Sojka M., Kolodziejczyk K., Milala J., Polyphenolic and basic chemical composition of black chokeberry industrial by-products, Ind. Crops Prod., 51, 77–86, 2013.
  34. 35. Cervenka L., Moisture adsorption characteristics of black currant, black elderberry and chokeberry samples at different temperatures, J. Food Process Eng., 34, 1419–1434, 2011.
  35. 36. Samoticha J., Wojdyło A., Lech K., The influence of different drying methods on chemical composition and antioxidant activity in chokeberries, LWT - Food Sci. Technol., 66, 484–489, 2016.
  36. 37. Tolic M.T., Jurčević I.L., Krbavčić I.P., Marković K., Vahčić N., Antioxidant properties of chokeberry products, Food Technol. Biotechnol., 53 (2), 171–179, 2015.
  37. 38. Miletić N., Popović B., Mitrović O., Kandić M., Leposavić A., Phenolic compounds and antioxidant capacity of dried and candied fruits commonly consumed in Serbia, Czech J. Food Sci., 32 (4), 360–368, 2014.
  38. 39. Han D., Wang W., Gong J., Ma Y., Li Y., Collagen hydroxyapatite based scaffolds for bone trauma and regeneration: recent trends and future perspectives, Nanomedicine, 19, 1689–1709, 2024.
  39. 40. Mulazzi M., Campodoni E., Bassi G., Montesi M., Panseri S., Bonvicini F., Gentilomi G.A., Tampieri A., Sandri M., Medicated hydroxyapatite/collagen hybrid scaffolds for bone regeneration and local antimicrobial therapy to prevent bone infections, Pharmaceutics, 13, 1090, 2021.
  40. 41. Becerra J., Rodriguez M., Leal D., Noris-Suarez K., Gonzalez G., Chitosan-collagen-hydroxyapatite membranes for tissue engineering, J. Mater. Sci.: Mater. Med., 33, 16, 2022.
  41. 42. Teng S.H., Lee E.J., Wang P., Shin D.S., Kim H.E., Three-layered membranes of collagen/hydroxyapatite and chitosan for guided bone regeneration, J. Biomed. Mater. Res. B Appl. Biomater., 87B, 132–138, 2008.
  42. 43. Kaynak Bayrak G., Demirtaş T. T., Gümüşderelioğlu M., Microwave-induced biomimetic approach for hydroxyapatitecoatings of chitosan scaffolds, Carbohydr. Polym., 157, 803–813, 2017.
  43. 44. Yılmaz B., Evis Z., Güldiken M., Biomimetic calcium phosphate coating of titanium alloy, Journal of the Faculty of Engineering and Architecture of Gazi University, 29 (1), 105–109, 2014.
  44. 45. Jankovic B., Marinovic-Cincovic M., Jankovic M., TG-DTA-FTIR analysis and isoconversional reaction profiles for thermal and thermo-oxidative degradation processes in black chokeberry, Chem. Pap., 70 (8), 1094–1105, 2016.
  45. 46. León-Mancilla B.H., Araiza-Téllez M.A., Flores-Flores J.O., Piña-Barba M.C., Physico-chemical characterization of collagen scaffolds for tissue engineering, J. Appl. Res. Technol., 14 (1), 77–85, 2016.
  46. 47. Woo H.W., Lee J.S., Characterization of electrospun Aronia melanocarpa fruit extracts loaded polyurethane nanoweb, Fashion Text., 8 (1), 1–14, 2021.
  47. 48. Hoang D.T., Thai H., Nguyen D.T.T., Vu M.T., Nguyen A.T., Nguyen T.X., Ngo Q.T.C., Nguyen C.T., Novel 3D Printed Biomaterials Based on Fish Scale Collagen and Plant Extracts and Its Hemostatic Efficacy, ChemistrySelect, 9 (e202402287), 1–16, 2024.
  48. 49. Mahmoudi F., Takallu S., Derakhshan M.A., Mirzaei E., A collagen-based bilayer nanocomposite scaffold for osteochondral tissue regeneration: integrating hydroxyapatite and cellulose nanocrystals, J. Mater. Sci. Mater. Med., 36 (88), 1–15, 2025.
  49. 50. Shagdarova B., Konovalova M., Zhuikova Y., Lunkov A., Zhuikov V., Khaydapova D., Il’ina A., Svirshchevskaya E., Varlamov V., Collagen/Chitosan Gels Cross-Linked with Genipin for Wound Healing in Mice with Induced Diabetes, Materials, 15 (15), 1–17, 2022.
  50. 51. Kaczmarek-Szczepańska B., Polkowska I., Małek M., The characterization of collagen-based scaffolds modified with phenolic acids for tissue engineering application, Sci. Rep., 13, 9966, 2023.