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Aronya ekstraktı içeren Kolajen/HA kompozit kemik greftinin üretimi ve karakterizasyonu

Production and characterization of Aronia extract containing Collagen/HA composite bone graft

Gökçe Kaynak Bayrak(İZMİR BAKIRÇAY ÜNİVERSİTESİ)0000-0001-7948-5087
Sayfa
1239–1248
Yayın Tarihi
2026-07-20
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Ayrıca, L929 fibroblast hücreleri ile gerçekleştirilen MTT analizleri, geliştirilen greftlerin sitotoksik olmadığını ve biyouyumlu yapıda olduğunu ortaya koymuştur.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Travma, kazalar veya onkolojik rezeksiyonlar sonucu ortaya çıkan kemik doku kayıpları, etkili doku replasmanı için gelişmiş kemik greft ikamelerinin geliştirilmesini gerekli kılmaktadır.
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Temel Bulgular & Veri:Biyouyumluluk ve osteogenezi destekleme gibi temel gerekliliklerin yanı sıra, rezeksiyon bölgesinde kalabilecek mikroskobik tümör hücrelerini baskılayabilecek antikanserojen fonksiyonelliğin kazandırılması, özellikle tümör sonrası rekonstrüksiyonda önemli bir terapötik üstünlük sağlayacaktır.
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Sonuç & Katkı:Ayrıca, L929 fibroblast hücreleri ile gerçekleştirilen MTT analizleri, geliştirilen greftlerin sitotoksik olmadığını ve biyouyumlu yapıda olduğunu ortaya koymuştur.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Kemik doku mühendisliği · biyomalzeme · kolajen

Özet

Travma, kazalar veya onkolojik rezeksiyonlar sonucu ortaya çıkan kemik doku kayıpları, etkili doku replasmanı için gelişmiş kemik greft ikamelerinin geliştirilmesini gerekli kılmaktadır. Biyouyumluluk ve osteogenezi destekleme gibi temel gerekliliklerin yanı sıra, rezeksiyon bölgesinde kalabilecek mikroskobik tümör hücrelerini baskılayabilecek antikanserojen fonksiyonelliğin kazandırılması, özellikle tümör sonrası rekonstrüksiyonda önemli bir terapötik üstünlük sağlayacaktır. Aronia melanocarpa, güçlü antioksidan, anti-inflamatuvar, antiproliferatif, antimikrobiyal ve antikanser aktiviteleriyle tanınan zengin biyoaktif fitokimyasal içeriğe sahip bir meyvedir. Bu çalışmada, ilk kez aronya ekstraktı kolajen–hidroksiapatit (Kol/HA) esaslı kompozit kemik greftlerinin üretiminde kullanılarak, yeni ve çok fonksiyonlu bir biyomalzeme platformu ortaya konmuştur. Greftler liyofilizasyon tekniğiyle üretilmiş ve genipin ile çapraz bağlanmıştır. Morfolojik, fizikokimyasal ve yapısal özellikleri; taramalı elektron mikroskobu (SEM), X-ışını kırınımı (XRD), ATR-FTIR ve şişme analizleri kullanılarak kapsamlı biçimde karakterize edilmiştir. Ayrıca, L929 fibroblast hücreleri ile gerçekleştirilen MTT analizleri, geliştirilen greftlerin sitotoksik olmadığını ve biyouyumlu yapıda olduğunu ortaya koymuştur.

Abstract

Bone tissue loss resulting from trauma, accidents, or oncological resections necessitates the development of advanced bone graft substitutes for effective tissue replacement. In addition to fundamental requirements such as biocompatibility and the ability to promote osteogenesis, the incorporation of anticarcinogenic functionality capable of suppressing residual microscopic tumor cells within the resection site would provide a substantial therapeutic advantage, particularly in post-tumor reconstruction. Aronia melanocarpa is a rich source of bioactive phytochemicals and has been widely recognized for its potent antioxidant, anti-inflammatory, antiproliferative, antimicrobial, and anticancer activities. In this study, for the first time, Aronia extract was incorporated into the fabrication of collagen–hydroxyapatite (Col/HA)-based composite bone grafts, introducing a novel multifunctional biomaterial platform. The grafts were fabricated via a lyophilization technique and cross-linked with genipin. Their morphological, physicochemical, and structural properties were comprehensively characterized using scanning electron microscopy (SEM), X-ray diffraction (XRD), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), and swelling analyses. Furthermore, cytocompatibility was confirmed by MTT assays using L929 fibroblast cells, demonstrating the non-cytotoxic nature of the developed grafts.

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Bu Makaleye Atıf Yap

Bayrak, G. K. (2026). Aronya ekstraktı içeren Kolajen/HA kompozit kemik greftinin üretimi ve karakterizasyonu. Gazi Üniversitesi Mühendislik Mimarlık Fakültesi Dergisi, Cilt: 41 Sayı: 2, 1239–1248. https://doi.org/10.17341/gazimmfd.1848993

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