The Effect of Single Dose Teriparatide Administration on the Early Healing Period of Bone Defects Created in the Rat Mandibular

Abstract

Introduction Bone grafts are widely used in the repair of bone tissue. In recent years, studies on the use of biomaterials have became popular in order to increase the efficiency of bone grafts. In this study, the effects of local teriparatide used in different doses on bone healing were evaluated. Materials - Methods In this study, 30 male Spraque-Dawley rats were used and they were divided into 5 groups, 6 each. A critical sized defect of 5 mm in diameter was created in the mandible. Defects in Group 1 were left empty. Particulate autograft was applied to the defects in Group 2. Group 3 was applied allograft. In Groups 4 and 5, 20 μg and 40 μg teriparatide were administered with the allograft, respectively. At the postoperative 4th week, the anesthesia dose was doubled and euthanasia was performed. The samples were taken out in one piece and evaluated histomorphometrically. Results While the amount of newly formed bone tissue (0.28±0.01 mm2) and the number of osteoblasts (41.67±1.56) were significantly lower in Group 1, these values were found in Group 5 (0.79±0.01 mm2), (77.72±3.06) is significantly higher. The findings in Group 4, in which teriparatide was used (0.72±0.01 mm2, 72±4.26), were significantly higher than Group 3 and Group 2. The amount of new bone formation (0.48 mm2) and the number of osteoblasts (54.17±1.99) were significantly higher in Group 3 than Group 2 (0.39±0.01 mm2, 50±1.4). Conclusion As a result, it was observed that teriparatide, which was used locally in two different doses, significantly increased bone healing