The Effects of Various Dental Restorative Materials on Neuroblastoma Cells

Abstract

Introduction: Our aim was to investigate the effects of restorative materials such as composite, compomer and glass ionomer, which are frequently used in dentistry, on SH-SY5Y neuroblastoma cells by evaluating oxidative stress parameters, pro- and anti-inflammatory cytokines and apoptosis markers. Materials and Method: Equa Forte, Dyract AP, Tokuyama Estelite P Quick, Omnichroma, Filtek Z250, SureFil SDR flow restorative materials were used in our study. SH-SY5Y neuroblastoma cells were cultured with restorative materials. Immunohistochemical staining was performed on the experimental groups with Anti-Bax and Anti-Caspase 9 antibodies. Then, ELISA technique was used to detect TNF-alpha, TGF-beta, IL-1-beta, IL-6, IL-10, LPO and CAT levels. One-way ANOVA analysis was used in the statistical evaluation of the obtained results (p<0.05). Results: In the light of the obtained data, it was observed that the dental filling materials were effective in increasing TGF-beta, IL-10, LPO and CAT levels, and decreasing TNF-alpha, IL-1-beta and IL-6 levels. Histological micrographs also supported the issues. When the H-score levels in the Caspase 9 labeled micrographs were evaluated, the mean of the control group was lower than the mean of the experimental groups. Conclusion: Our study shows that biocompatibility cannot be explained by looking at a single cause. Biocompatibility varies with material content, residual monomer amount and solubility. Although all experimental groups have cytotoxic effects, the least effect is seen in the glass ionomer group.