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Dergiler / Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi / 2022 / Cilt: 23 Sayı: 2

Türkiye'de Hyphantria cunea larvalarından izole edilen iki yeni entomopatojenik Beauveria bassiana suşunun karakterizasyonu ve biyolojik aktivitesi

Characterization and biological activity of two new entomopathogenic Beauveria bassiana strains isolated from Hyphantria cunea larvae in Turkey

Zeynep Bayramoğlu(RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ, PAZAR MESLEK YÜKSEKOKULU)0000-0001-6994-1106
Sayfa
209–216
Yayın Tarihi
2022-10-28
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Sonuç olarak, HC-Z1 ve HC-Z2 suşlarının potansiyel olarak H. cunea'ya karşı biyolojik kontrol etmeni olarak kullanılabileceği sonucuna varılabilir.

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Amaç & Hipotez:Bu çalışmada, Hyphantria cunea (Amerikan beyaz kelebeği) larvalarından izole edilen iki fungus suşunun (HC-Z1 ve HC-Z2), H. cunea larvalarına karşı biyokontrol etmeni olarak potansiyelleri araştırıldı.
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Temel Bulgular & Veri:Morfolojik ve moleküler (ITS1-5.8S-ITS2 bölgesinin dizileri) karakterizasyonlarına göre suşlar Beauveria bassiana (HC-Z1: OP415530 ve HC-Z2: OP415531) olarak tanımlandı.
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Sonuç & Katkı:Sonuç olarak, HC-Z1 ve HC-Z2 suşlarının potansiyel olarak H. cunea'ya karşı biyolojik kontrol etmeni olarak kullanılabileceği sonucuna varılabilir.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Hyphantria cunea · Beauveria bassiana · biyolojik mücadele

Özet

Bu çalışmada, Hyphantria cunea (Amerikan beyaz kelebeği) larvalarından izole edilen iki fungus suşunun (HC-Z1 ve HC-Z2), H. cunea larvalarına karşı biyokontrol etmeni olarak potansiyelleri araştırıldı. Morfolojik ve moleküler (ITS1-5.8S-ITS2 bölgesinin dizileri) karakterizasyonlarına göre suşlar Beauveria bassiana (HC-Z1: OP415530 ve HC-Z2: OP415531) olarak tanımlandı. İki fungus suşunun biyolojik aktivitelerini belirlemek için H. cunea larvalarının 3. evresinde konsantrasyon yanıt testi (1 × 104-8 konidia/ml) yapıldı. Ayrıca, iki B. bassiana suşu , 1 × 107 konidia/ml konsantrasyonu H. cunea larvalarının beş evresi (1-5.) üzerinde test edildi. Her iki suş da H. cunea'nın birinci evre larvalarına karşı 7 gün içinde %96’nın üzerinde ölüm oranı oluşturdu. Üçüncü evre H. cunea larvalarına karşı HC-Z1 ve HC-Z2 suşlarının LC50 değerleri sırasıyla 0.6 × 104 ve 1.2 × 104 konidia/ml olarak hesaplandı. LT50 değerleri ise HC-Z1 ve HC-Z2 suşları için sırasıyla 2.82 ve 3.44 gün olarak belirlenmiştir. Sonuç olarak, HC-Z1 ve HC-Z2 suşlarının potansiyel olarak H. cunea'ya karşı biyolojik kontrol etmeni olarak kullanılabileceği sonucuna varılabilir.

Abstract

In this study, two fungal strains (HC-Z1 and HC-Z2) from Hyphantria cunea (fall webworm) larvae were evaluated for their potential as biocontrol agents against the H. cunea larvae. Based on morphological and molecular (ITS1-5.8S-ITS2 region) characterization, the strains were identified as Beauveria bassiana (HC-Z1: OP415530 and HC-Z2: OP415531). To determine the biological activities of the two fungal strains, a concentration-response assay (1 × 104-8 conidia/ml) was performed against third stage H. cunea larvae. In addition, two B. bassiana strains were tested on five (1-5) larval stages at 1 × 107 conidia/ml concentration. Both isolates produced mortalities over 96% within 7 days for the first larval stage of H. cunea. The LC50 and LT50 of HC-Z1 and HC-Z2 strains against third instar H. cunea larvae were calculated as 0.6 × 104 and 1.2 × 104 conidia/ml, respectively. LT50 values were obtained in 2.82 and 3.44 days for HC-Z1 and HC-Z2 isolates, respectively. As a result, it can be concluded that HC-Z1 and HC-Z2 strains can potentially be used as biological control agents against H. cunea.

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Bu Makaleye Atıf Yap

Bayramoğlu, Z. (2022). Türkiye'de Hyphantria cunea larvalarından izole edilen iki yeni entomopatojenik Beauveria bassiana suşunun karakterizasyonu ve biyolojik aktivitesi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, Cilt: 23 Sayı: 2, 209–216. https://doi.org/10.17474/artvinofd.1181151

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