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Dergiler / Marmara Medical Journal / 2023 / Cilt: 36 - Sayı: 1

Effect of whey protein derivatives on cell viability, cell migration and cell cycle phases in MCF-7 cells

F. Tutku AKSOY
Ayse Mine YILMAZ
Gokhan BICIM
A. Suha YALCIN
Sayfa
39–45
Yayın Tarihi
2023-01-31
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Conclusion: Our results showed that whey protein derivatives applied to MCF-7 cells are cytotoxic and may be useful in breast cancer treatment.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: This study aimed to obtain protein derivatives after treatment of whey proteins with hazelnut oil and olive oil and determined their effects on MCF-7 cells.
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Temel Bulgular & Veri:Materials and Methods: Whey proteins obtained from 6% whey powder were treated with hazelnut oil (HO) and olive oil (OO) at a protein to lipid ratio of 1:10 at 60 ̊C for 120 minutes.
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Sonuç & Katkı:Conclusion: Our results showed that whey protein derivatives applied to MCF-7 cells are cytotoxic and may be useful in breast cancer treatment.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Whey proteins · Oleic acid · Cell survival

Abstract

Objective: This study aimed to obtain protein derivatives after treatment of whey proteins with hazelnut oil and olive oil and determined their effects on MCF-7 cells. Materials and Methods: Whey proteins obtained from 6% whey powder were treated with hazelnut oil (HO) and olive oil (OO) at a protein to lipid ratio of 1:10 at 60 ̊C for 120 minutes. The protein derivatives formed with whey protein and HO or OO were applied to MCF-7 cancer cells and healthy fibroblasts. The effects of protein derivatives on cell viability, apoptosis, reactive oxygen species (ROS) production, wound healing, cell cycle phase distribution and cell cycle related proteins Akt and p21(Waf1/Cip1) expressions were investigated. Results: Cell viability decreased significantly after 24 h of incubation with WP:OO. The percentage of apoptotic or necrotic cells varied between 5-10% and no statistically significant effect was observed. There was no statistically significant difference in ROS production and colony formation between controls and WP:HO or WP:OO groups. Treatment of cells with WP:OO for 24 h significantly decreased cell migration compared to the control group. G2/M phase was significantly suppressed in WP:OO group compared to the control group. WP:OO also increased the expression of p21(Waf1/Cip1) significantly when compared with the control group. Conclusion: Our results showed that whey protein derivatives applied to MCF-7 cells are cytotoxic and may be useful in breast cancer treatment.

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Bu Makaleye Atıf Yap

AKSOY, F. T., YILMAZ, A. M., BICIM, G., YALCIN, A. S. (2023). Effect of whey protein derivatives on cell viability, cell migration and cell cycle phases in MCF-7 cells. Marmara Medical Journal, Cilt: 36 - Sayı: 1, 39–45. https://ajindex.com/articles/879992

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Kaynakça (2)

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