Dergiler / International Journal of Nature and Life Sciences / 2022 / Cilt: 6 - Sayı: 2

Detection of Cadmium Genotoxicity on Fatty Acids Desaturase-2 Genes in Safflower (Carthamus tinctorius)

Sayfa
99–117
Yayın Tarihi
2022-12-01
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

In conclusion, the obtained data showed that FAD2 genes in safflower cultivars not only in the conversion of fatty acids but also play a critical role in defense against cadmium heavy metal stress.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Environmental pollution negatively affects the life activities of the living things of the environment and causes structural damages on the all living and inanimate things.
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Temel Bulgular & Veri:In this study, in experimental groups prepared at different cadmium concentrations, the negative/genotoxic effects of cadmium on the mRNA expression levels of the FAD2 (FAD2-6, FAD2-7, FAD2-11) genes, which is responsible for the conversion of oleic acid to linoleic acid in different safflower varieties (Balci, Bdyas-04, Linas and Asol) has been determined by quantitative Real-Time PCR method.
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Sonuç & Katkı:In conclusion, the obtained data showed that FAD2 genes in safflower cultivars not only in the conversion of fatty acids but also play a critical role in defense against cadmium heavy metal stress.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Safflower · Cadmium · FAD2 Gene

Abstract

Environmental pollution negatively affects the life activities of the living things of the environment and causes structural damages on the all living and inanimate things. The extent of the damage caused by human-made pollution to nature and the environment is increasing day by day. Heavy metal pollution is one of the most important causes of environmental pollution. Cadmium, which is one of the most important pollutants, is a highly toxic metal and is not used by living things, even in trace amounts. Cadmium is also a very toxic heavy metal for plants. As with other heavy metals, it triggers oxidative stress by increasing the production of reactive oxygen species in plant cells, causing DNA damage and abnormalities in DNA and RNA production. Safflower is a very important oil plant with high economic value and intensive use as a raw material in many sectors. In this study, in experimental groups prepared at different cadmium concentrations, the negative/genotoxic effects of cadmium on the mRNA expression levels of the FAD2 (FAD2-6, FAD2-7, FAD2-11) genes, which is responsible for the conversion of oleic acid to linoleic acid in different safflower varieties (Balci, Bdyas-04, Linas and Asol) has been determined by quantitative Real-Time PCR method. As a result, it has been detected that the decrease firstly in the expression of FAD2 genes at increasing cadmium concentrations in all cultivars. And also, re-increase at 160 and 320 mg L-1 which can be considered as critical points, have been accepted as an indication that the defense mechanism against stress is activated and FAD2 genes play a role in the defense against stress. In conclusion, the obtained data showed that FAD2 genes in safflower cultivars not only in the conversion of fatty acids but also play a critical role in defense against cadmium heavy metal stress.

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Bu Makaleye Atıf Yap

BÖLÜKBAŞI, E., ARAS, S. (2022). Detection of Cadmium Genotoxicity on Fatty Acids Desaturase-2 Genes in Safflower (Carthamus tinctorius). International Journal of Nature and Life Sciences, Cilt: 6 - Sayı: 2, 99–117. https://ajindex.com/articles/868745

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Kaynakça (10)

  1. Ahlawat, I. P. S. (2008). Agronomy-Rabi Crops Safflower. Division of Agronomy Indian Agricultural Research Institute, 10, New Delhi, India. Anonymous. 2013. https://www.agroscope.admin.ch/agroscope/en/home/topics/plantproduction /fieldcrops/kulturarten/alternative-kulturpflanzen/saflor.html (date of access: 26.06.2018).
  2. Babaoglu, M. (2007). Safflower and its Cultivation. Trakya Agricultural Research Institute, Edirne. Bayrak, A. 1997. Investigation of fatty acid composition of summer-winter Safflower (Carthamus tinctorius L.) cultivars and lines tested in Ankara and Şanlıurfa. Journal of Food, 22(4), 269-277.
  3. Bolukbasi, E. (2021). Expression analysis of some stress-related genes induced by cadmium on tomato (Solanum lycopersicum L.) plants. Hittite Journal of Science and Engineering, 8(4), 339-345. doi.org/10.17350/HJSE19030000247 Bolukbasi, E. (2022). Analysis of Genetic and Epigenetic Effects of Sunflower (Helianthus annuus L.) Seedlings in Response to Copper Stress. Fresenius Environmental Bulletin, 31(4), 4596-4602.
  4. Buyuk, I., Bolukbasi, E., & Aras, E. S. (2016). Expression of CtFAD2 gene for early selection in safflower oleic linoleic oil content. Journal of Animal and Plant Sciences, 26(5), 1383-1388.
  5. Cao, S., Zhou, X. R., Wood, C. C., Green, A. G., Singh, S. P., Liu, L., & Liu, Q. (2013). A large and functionally diverse family of FAD2 genes in safflower (Carthamus tinctorius L.). BMC Plant Biology, 13(5), 1-18.
  6. Cobbett, C., & Goldsbrough, P. B. (2002). Phytochelatins and Metallothioneins: Roles in Heavy Metal Detoxification and Homeostasis. Annual Review of Plant Biology, 53, 159-182.
  7. Davis, P. H. (1975). Flora of Turkey and the East Aegeans Islands (Vol: 5), Edinburg: Edinburg University Press.
  8. Dietz, K. J. (1999). Free Radicals and Reactive Oxygen Species as Mediator of Heavy Metal Toxicity in Plants, pp. 73-79, In: Prasad, M.N.V., Hagemeyer, J., (Eds), Berlin, Springer. Dunnett, C. W. 1955. A multiple comparison procedure for comparing several treatments with a control. Journal of the American Statistical Association, 50, 1096-1121.
  9. Feng, J., Dong, Y., Liu, W., He, Q., & Daud, M. K. (2017). Genome wide identification of membrane-bound fatty acid desaturase genes in Gossypium hirsutum and their expressions during abiotic stress. Scientific Reports, 7, 1-12. https://doi:10.1038/srep45711
  10. Gautam, S., Anjani, K., & Srivastava, N. (2016). In vitro evaluation of excess copper affecting seedlings and their biochemical characteristics in Carthamus tinct