KaydedilenlerDerginizi ekleyin

Dergiler / International Journal of Life Sciences and Biotechnology / 2022 / Cilt: 5 - Sayı: 3

Antitumor Activity of Etoposide, Puerarin, Galangin and Their Combinations in Neuroblastoma Cells

Çağatay OLTULU
Melek AKINCI
Elvan BAKAR
Sayfa
407–423
Yayın Tarihi
2022-12-15
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

It is concluded that single therapy of galangin and puerarin may be promising in the treatment of neuroblastoma.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Neuroblastoma is a disease that is observed in early childhood, originating from the sympathetic nervous system and very difficult to treat.
📊
Temel Bulgular & Veri:Etoposide, a topoisomerase 2 inhibitor, is one of the agents used in cancer treatment.
💡
Sonuç & Katkı:It is concluded that single therapy of galangin and puerarin may be promising in the treatment of neuroblastoma.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Etoposide · galangin · puerarin

Abstract

Neuroblastoma is a disease that is observed in early childhood, originating from the sympathetic nervous system and very difficult to treat. Etoposide, a topoisomerase 2 inhibitor, is one of the agents used in cancer treatment. Galangin and puerarin are plant-based antioxidants with anticancer properties. Since apoptosis induction is one of the methods used in cancer treatment, the evaluation of the mRNA expression levels of apoptosis-related genes is aimed in our study. We investigated the effects of etoposide and galangin/puerarin combination therapy on neuroblastoma and astrocyte cells’ apoptotic process in this study. IC50 dose was determined by MTT test in neuroblastoma and healthy astrocyte lines. Apoptosis-related mRNA gene expressions (topoisomerase 1 and 2α, BAX, p53, TNFα, BCL-2, caspase 3, IL-1, caspase 9) were evaluated in astrocyte and neuroblastoma cells at the dose of neuroblastoma IC50. It was resulted that in all groups, the neuroblastoma IC50 dose was lower than the healthy astrocyte cell IC50 dose and while an increase in apoptotic mRNA expressions was observed in the neuroblastoma cancer line, the mRNA expression changes in the astrocyte cell line did not cause apoptosis. Etoposide combinations antiproliferative effect was decreased relative to etoposide group. It is concluded that single therapy of galangin and puerarin may be promising in the treatment of neuroblastoma.

🔖

Bu Makaleye Atıf Yap

OLTULU, Ç., AKINCI, M., BAKAR, E. (2022). Antitumor Activity of Etoposide, Puerarin, Galangin and Their Combinations in Neuroblastoma Cells. International Journal of Life Sciences and Biotechnology, Cilt: 5 - Sayı: 3, 407–423. https://ajindex.com/articles/868722

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (16)

  1. Urbani, A., et al., A proteomic investigation into etoposide chemo‐resistance of neuroblastoma cell lines. Proteomics, 2005. 5(3): p. 796-804
  2. Steliarova-Foucher, E., et al., International incidence of childhood cancer, 2001-10: a population-based registry study. Lancet Oncol, 2017. 18(6): p. 719-731
  3. Maris, J.M., et al., Neuroblastoma. Lancet, 2007. 369(9579): p. 2106-20
  4. Pinto, N.R., et al., Advances in Risk Classification and Treatment Strategies for Neuroblastoma. J Clin Oncol, 2015. 33(27): p. 3008-17
  5. Berger, S.J., et al., Green tea constituent (--)-epigallocatechin-3-gallate inhibits topoisomerase I activity in human colon carcinoma cells. Biochem Biophys Res Commun., 2001. 288(1): p. 101-5
  6. Nitiss, J.L., Targeting DNA topoisomerase II in cancer chemotherapy. Nat Rev Cancer, 2009. 9(5): p. 338-50
  7. Karpinich, N.O., et al., The course of etoposide-induced apoptosis from damage to DNA and p53 activation to mitochondrial release of cytochrome c. J Biol Chem, 2002. 277(19): p. 16547-52
  8. Janicke, R.U., D. Sohn, and K. Schulze-Osthoff, The dark side of a tumor suppressor: anti-apoptotic p53. Cell Death Differ, 2008. 15(6): p. 959-76
  9. Yonish-Rouach, E., The p53 tumour suppressor gene: a mediator of a G1 growth arrest and of apoptosis. Experientia, 1996. 52(10-11): p. 1001-7
  10. Heo, M.Y., S.J. Sohn, and W.W. Au, Anti-genotoxicity of galangin as a cancer chemopreventive agent candidate. Mutat Res., 2001. 488(2): p. 135-150
  11. Bacanli, M., A.A. Basaran, and N. Basaran, The antioxidant, cytotoxic, and antigenotoxic effects of galangin, puerarin, and ursolic acid in mammalian cells. Drug Chem Toxicol, 2017. 40(3): p. 256-262
  12. Zhang, H.T., et al., Galangin induces apoptosis of hepatocellular carcinoma cells via the mitochondrial pathway. World J Gastroenterol, 2010. 16(27): p. 3377-84
  13. Zeng, H., et al., Galangin-induced down-regulation of BACE1 by epigenetic mechanisms in SH-SY5Y cells. Neuroscience, 2015. 294: p. 172-81
  14. Zhang, W., et al., Galangin induces B16F10 melanoma cell apoptosis via mitochondrial pathway and sustained activation of p38 MAPK. Cytotechnology, 2013. 65(3): p. 447-55
  15. Zou, W.-W. and S.-P. Xu, Galangin inhibits the cell progression and induces cell apoptosis through activating PTEN and Caspase-3 pathways in retinoblastoma. Biomed Pharmacother., 2018. 97: p. 851-863
  16. Li, J., et al., Anti-colorectal cancer biotargets and biological mechanisms of puerarin: Study of molecular networks. Eur J Phar