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Dergiler / Natural and Engineering Sciences / 2017 / Cilt: 2 - Sayı: 3

Assessing DNA Barcodes for Identification of Pufferfish Species (Tetraodontidae) in Turkish Marine Waters

Cemal Turan
Mevlüt Gürlek
Deniz Ergüden
Ali Uyan
Serpil Karan
Servet Ahmet Doğdu
Sayfa
55–66
Yayın Tarihi
2017-12-19
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The number of detected different haplotypes were 10 out of 23 sequences, and haplotype diversity was found to be 1.000.

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Amaç & Hipotez:In Turkish marine waters, pufferfish belongs to Tetraondontidae family are represented with 8 species, Lagocephalus lagocephalus, L. sceleratus, L. spadiceus, L. suezensis, L. guentheri, Sphoeroides pachygaster, Torquigener flavimaculosus and Tylerius spinosissimus.
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Temel Bulgular & Veri:COI contained 189 variable and 337 conservative nucleotides of which 183 were parsimony informative over 526 bp.
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Sonuç & Katkı:The number of detected different haplotypes were 10 out of 23 sequences, and haplotype diversity was found to be 1.000.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Pufferfish · catch amount · Aegean Sea

Abstract

In Turkish marine waters, pufferfish belongs to Tetraondontidae family are represented with 8 species, Lagocephalus lagocephalus, L. sceleratus, L. spadiceus, L. suezensis, L. guentheri, Sphoeroides pachygaster, Torquigener flavimaculosus and Tylerius spinosissimus. DNA barcoding can be useful in the assessment of cryptic or morphologically similar species of identification which is widespread in marine environment. DNA barcode identification of the eight puffer species of the Tetraodontidae family in Turkish marine waters were examined by using mtDNA sequencing of the amplified partial mitochondrial cytochrome c oxidase I (COI) gene. COI contained 189 variable and 337 conservative nucleotides of which 183 were parsimony informative over 526 bp. Mean genetic diversity all species was found 0.18164. The highest and lowest nucleotide divergence was observed L.spadiceus (0.0022) and between L. sceleratus, L. suezensis, L. lagocephalus, L. guentheri and S. pachygaster (0.0000) respectively. The number of detected different haplotypes were 10 out of 23 sequences, and haplotype diversity was found to be 1.000.

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Bu Makaleye Atıf Yap

Turan, C., Gürlek, M., Ergüden, D., Uyan, A., Karan, S., Doğdu, S. A. (2017). Assessing DNA Barcodes for Identification of Pufferfish Species (Tetraodontidae) in Turkish Marine Waters. Natural and Engineering Sciences, Cilt: 2 - Sayı: 3, 55–66. https://ajindex.com/articles/793253

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Kaynakça (13)

  1. Avise, J.C. 1994.
  2. Molecular Markers, Natural History and Evolution. Chapman and Hall, New York, USA, 511 pp. El-Sayed, M., Yacout, G. A., El-Samra, M., Ali, A., & Kotb, S. M. 2003. Toxicity of the Red Sea pufferfish Pleuranacanthus sceleratus “El-Karad”. Ecotoxicology and environmental safety, 56(3), 367-372.
  3. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39(4), 783-791.
  4. Froese, R., & Pauly, D. 2016. FishBase. Worldwide web electronic publication. 2014.
  5. Hauser, L., Turan, C., Carvalho, G.R. 2001. Haplotype frequency distribution and discriminatory power of two mtDNA fragments in a marine pelagic teleost (Atlantic herring, Clupea harengus). Heredity, 87: 621–630.
  6. Hebert, P.D.N., Cywinska, A., Ball, S.L., deWaard, J.R. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B 270: 313–321.
  7. Matsuura, K. 2015. Taxonomy and systematics of tetraodontiform fishes: a review focusing primarily on progress in the period from 1980 to 2014. Ichthyological Research, 62(1), 72-113.
  8. Mohindra, V., Singh, R.K., Palanichamy, M., Ponniah, A.G., Lal, K.K. 2007. Genetic identification of three species of the genus Clarias using allozyme and mitochondrial DNA markers. Journal of Applied Ichthyology, 23:104–109.
  9. Meyer, A. 1993. Evolution of mitochondrial DNA in fishes. In: Biochemistry and Molecular Biology of Fishes. Elsevier Science Publishers, 2: 1-38.
  10. Nei, M., &Kumar, S. 2000. Molecular evolution and phylogenetics. Oxford university press. Oyaizu, M., Fujimoto, Y., Takenaga, F., & Itoh, S. 2000. Fatty acid composition of total lipids in puffer fish meat. Food Preservation Science, 26(6), 333-338.
  11. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95-98.
  12. Farrag, M. M. S. 2014. Fisheries and Biological studies on Lessepsian pufferfish, Lagocephalus sceleratus (Gmelin, 1789) (Family: Tetraodontidae) in the Egyptian Mediterranean waters. Faculty of Sciences Al-Azhar University (Assiut), Egypt. Farrag, M., El-Haweet, A. A., and Moustafa, M. A. 2016. Occurrence of puffer fishes (Tetraodontidae) in the eastern Mediterranean, Egyptian coast-filling in the gap. BioInvasions Record, 5(1). Keskin, E., & Atar, H. H. 2013. DNA barcoding commercially important fish species of Turkey. Molecular Ecology Resources, 13(5), 788-797.
  13. Kumar, S., Stecher, G., & Tamura, K. 2016. MEGA7: Molecular Evo