Neuroprotective effect of Hypericum perforatum extract against aluminum-maltolate induced toxicity in SH-SY5Y cells

To determine synergistic and antagonistic effects of H. perforatum extract content is important to examine their specific effects of together with hyperforin, which is a phytochemical produced by some of the members of the plant genus Hypericum, to discover new therapeutic agents against neurodegeneration.

📌 3 Maddede Bu Makale

🎯

Amaç & Hipotez:Alzheimer's disease is multi-component neurodegenerative disorder.

📊

Temel Bulgular & Veri:In this study, it is aimed to consider the antioxidant potential of Hypericum perforatum extract against neurotoxicity caused by Aluminum-maltolate (Al(mal)3) and its effects on APP gene expression.

💡

Sonuç & Katkı:To determine synergistic and antagonistic effects of H. perforatum extract content is important to examine their specific effects of together with hyperforin, which is a phytochemical produced by some of the members of the plant genus Hypericum, to discover new therapeutic agents against neurodegeneration.

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Alzheimer · Oxidative stress · Aluminum maltolate

Abstract

Alzheimer's disease is multi-component neurodegenerative disorder. Oxidative stress disrupts regular functioning of metabolism in early-onset Alzheimer's disease. It causes Tau phosphorylation, formation of neurofibrillary tangle and neuron reduction. Due to intense binding of phosphorylated amino acids to aluminum, it induces self-assembly and deposition of high degree of phosphorylated cytoskeletal proteins, such as microtubule and neurofilament-associated proteins. In this study, it is aimed to consider the antioxidant potential of Hypericum perforatum extract against neurotoxicity caused by Aluminum-maltolate (Al(mal)3) and its effects on APP gene expression. Four different groups were determined to observe the impact of H. perforatum extract. After the incubation of the cells for 24 hours, only the medium was placed in the first group as control. 500 μM Al(mal)3 was added to the second group of cells. 20 μg mL-1 Hypericum perforatum extract was added to the third group. For the fourth group, 20 μg mL-1 Hypericum perforatum extract and 500 μM Al(mal)3 were added. While Al(mal)3 increased total antioxidant status levels in SH-SY5Y human neuroblastoma cells, H. perforatum extract significantly inhibited Al(mal)3 induced oxidative stress. On the other hand, H. perforatum extract significantly decreased APP gene expression levels depending on Al(mal)3 toxicity in SH-SY5Y cells. According to these results, H. perforatum extract significantly inhibited Al(mal)3 neurotoxicity against SH-SY5Y cells. To determine synergistic and antagonistic effects of H. perforatum extract content is important to examine their specific effects of together with hyperforin, which is a phytochemical produced by some of the members of the plant genus Hypericum, to discover new therapeutic agents against neurodegeneration.

Anahtar kelimeler

Alzheimer Oxidative stress Aluminum maltolate Neurodegeneration

🔖

Bu Makaleye Atıf Yap

AKKUŞ, R. Y., BİTMEZ, B., GÜLTEKİN, S. K., ALBAYRAK, İ. G., ÖZEN, F., DEVECİ, Y., SICAK, Y., AKALIN, E., PİRHAN, A. F., ARSLAN, B. A. (2022). Neuroprotective effect of Hypericum perforatum extract against aluminum-maltolate induced toxicity in SH-SY5Y cells. International Journal of Science Letters, Cilt: 4 - Sayı: 2, 277–291. https://ajindex.com/articles/768446

📄 Tam Metin PDF Okuyucu

Yeni Sekmede Aç ↗

↗Yeni Sekme

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (10)

Abdpour, S., Jalili-Baleh, L., Nadri, H., Forootanfar, H., Bukhari, S.N.A. et al. (2021). Chromone derivatives bearing pyridinium moiety as multi-target-directed ligands against alzheimer’s disease, Bioorganic Chemistry, 110. doi:10.1016/J.BIOORG.2021.104750 Akiyama, H., Barger, S., Barnum, S., Bradt, B., Bauer, J. et al. (2000). Inflammation and Alzheimer’s Disease, Neurobiology of Aging, 21:383–421.
Altmann, P., Cunningham, J., Dhanesha, U., Ballard, M., Thompson, J., Marsh, F. (1999). Disturbance of cerebral function in people exposed to drinking water contaminated with aluminium sulphate: Retrospective study of the camelford water ıncident, British Medical Journal, 319:807–811.
Arslan, B.A., Isik, F.B., Gur, H., Ozen, F., Catal, T. (2017). Apoptotic effect of Nigella sativa on human lymphoma U937 cells, Pharmacognosy Magazine, 13:S628–S632. Atasever-Arslan, B., Y. Ozer, F. Ozen, Y. Diler, and A.D. Yalcin, (2020). Tumor necrosis factor alpha gene Rs1799724 polymorphism in Alzheimer’s Disease, General Physiology and Biophysics, 39:595–599.
Aydin, D., Weyer, S.W., Müller, U.C. (2012). Functions of the APP gene family in the nervous system: Insights from mouse models, Experimental Brain Research, 217:423–434.
Collin, R.W.J., Van Strien, D., Leunissen, J.A.M., Martens, G.J.M. (2004). Identification and expression of the first nonmammalian amyloid-beta precursor-like protein APLP2 in the amphibian Xenopus laevis, European Journal of Biochemistry, 271:1906–1912.
Deveci, Y., Gunal-Sadik, G., Akalın-Urusak, E., Kuşoğlu-Gültekin, S., Yanık A., Atasever-Arslan, B. (2021). Anti-diabetic effects of Berberis cretica extract in INS-1E cells, International Journal of Science Letters, 3(2):121-128.
Dinamarca, M.C., Cerpa, W., Garrido, J., Hancke, J.L., Inestrosa, N.C. (2006). Hyperforin prevents β-Amyloid neurotoxicity and spatial memory impairments by disaggregation of Alzheimer’s Amyloid-β-deposits, Molecular Psychiatry, 11:1032–1048.
Exley, C. (1999). A molecular mechanism of aluminium-induced Alzheimer’s Disease? Journal of Inorganic Biochemistry, Journal of Inorganic Biochemistry, 76(2): 133–140.
Fleszar, M.G., Wiśniewski, J., Zboch, M., Diakowska, D., Gamian, A., Krzystek-Korpacka, M. (2019). Targeted metabolomic analysis of nitric oxide/l-arginine pathway metabolites in dementia: Association with pathology, severity, and structural brain changes, Scientific Reports, 9:1–15.
Garg, A.D., Krysko, D.V., Vandenabeele, P., Agostinis, P. (2012). Hypericin-based photody