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AI InsightSonuç olarak, nuc geni toplam 100 KPS izolatının 72 (%72)’isinde saptandı ve bu izolatlar S.aureus (%72, n=72; 44’ü süt orjinli, 28’i ise peynir orjinli) olarak değerlendirildi.100 KPS izolatında mecA geni ise 15 izolatta (%15, n=15; 4 süt ve 11 peynir orjinli) saptandı. mecA geni saptanan bu 15 izolat MRKPS olarak belirlendi.
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Özet
Today, MRSA is among the most important causes of antimicrobial-resistant health care-associated infections worldwide. The aim of the present study, 100 coagulase positive staphylococci (CPS) isolates recovered from raw cow milk and different kind of cheese samples, collected from different part of Samsun province, Turkey, using classic culture technique were determined for Staphylococcus aureus (S. aureus) and methicillin-resistant (MR) properties. For S. aureus determination of the isolates, nuc gene (S. aureus specific gene) was detected, and to determination of methicillin resistance properties of the isolates, mecA gene was determined in the isolates using PCR assay technique. As a result; nuc gene was detected in 72 (72%) (44 of which milk and 28 of which cheese origin) out of 100 CPS. In the 100 CPS isolates, mecA gene was detected 15 (15%, n=15; 4 milk and 11 cheese origin) CPS isolates, and the isolates were evaluated as MRCPS. The number of both nuc and mecA present isolates were 5 (4 milk and 1 cheese origin). These 5 isolates were evaluated as MRSA (5%). The remaining 10 (%10) containing mecA isolates were evaluated as MRCPS. In conclusion, raw cow milk and cheese samples have a potential health risk for mainly S. aureus and then coagulase positive staphylococci. In addition to this, analyzed samples also contaminated with MRSA or MRCPS isolates, become a big challenge for human health. Günümüzde MRSA, dünya genelinde antimikrobiyel dirençlilik ile ilgili sağlık problemlerinin en önemli nedenleri arasında yer almaktadır. Bu çalışmada, çiğ inek sütü (n=50) ve çeşitli tip peynirlerden (n=50) klasik kültür tekniği ile izole edilen toplam 100 koagulaz pozitif staphylococcus (KPS) izolatlarında S. aureus’un belirlenmesi ve metisiline dirençlilik (MR) özelliklerinin moleküler yöntemle saptanması amacıyla analiz edildi. S.aureus’un saptanması için nuc, metisiline dirençlilik özelliklerini saptanması ise mecA gen varlıkları PZR tekniği kullanılarak saptandı. Sonuç olarak, nuc geni toplam 100 KPS izolatının 72 (%72)’isinde saptandı ve bu izolatlar S.aureus (%72, n=72; 44’ü süt orjinli, 28’i ise peynir orjinli) olarak değerlendirildi.100 KPS izolatında mecA geni ise 15 izolatta (%15, n=15; 4 süt ve 11 peynir orjinli) saptandı. mecA geni saptanan bu 15 izolat MRKPS olarak belirlendi. Çalışmada nuc ve mecA genlerinin her ikisinin de bulunduğu izolat sayısı ise 5 (4 süt, 1 peynir orjinli) olup, bu izolatlar MRSA (%5) olarak değerlendirilirken, geriye kalan 10 izolat ise MRKPS (%10) olarak değerlendirildi. Bu çalışma bulguları çerçevesinde, Samsun ilinde tüketime sunulan peynirlerin ve çiğ inek sütlerinin başta S. aureus olmak üzere koagulaz pozitif stafilokoklarla kontamine olduğu, ayrıca analiz edilen örneklerin halk sağlığı yönünden çok önemli bir tehdit olan MRSA ve MRCPS ile de kontamine olduğu saptandı.
Abstract
Today, MRSA is among the most important causes of antimicrobial-resistant health care-associated infections worldwide. The aim of the present study, 100 coagulase positive staphylococci (CPS) isolates recovered from raw cow milk and different kind of cheese samples, collected from different part of Samsun province, Turkey, using classic culture technique were determined for Staphylococcus aureus (S. aureus) and methicillin-resistant (MR) properties. For S. aureus determination of the isolates, nuc gene (S. aureus specific gene) was detected, and to determination of methicillin resistance properties of the isolates, mecA gene was determined in the isolates using PCR assay technique. As a result; nuc gene was detected in 72 (72%) (44 of which milk and 28 of which cheese origin) out of 100 CPS. In the 100 CPS isolates, mecA gene was detected 15 (15%, n=15; 4 milk and 11 cheese origin) CPS isolates, and the isolates were evaluated as MRCPS. The number of both nuc and mecA present isolates were 5 (4 milk and 1 cheese origin). These 5 isolates were evaluated as MRSA (5%). The remaining 10 (%10) containing mecA isolates were evaluated as MRCPS. In conclusion, raw cow milk and cheese samples have a potential health risk for mainly S. aureus and then coagulase positive staphylococci. In addition to this, analyzed samples also contaminated with MRSA or MRCPS isolates, become a big challenge for human health.