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Dergiler / Eurasian Journal of Biological and Chemical Sciences / 2020 / Cilt: 3 - Sayı: 2

A Validated HPLC-UV Method for Determination of Dopamine HCl in Injectable Solutions

Beyza Sultan AYDIN
İbrahim BULDUK
Sayfa
116–120
Yayın Tarihi
2020-12-29
DOI
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This method can be used successfully in pharmaceutical formulations for routine analysis.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Dopamine is a neurotransmitter that acts in the nervous system as a chemical messenger, which helps specific neurons to interact with each other.
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Temel Bulgular & Veri:A simple, precise, accurate HPLC method has been developed and validated for determination of Dopamine HCl (DOPHCl) in pharmaceutical formulations.
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Sonuç & Katkı:This method can be used successfully in pharmaceutical formulations for routine analysis.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Dopamin HCl · HPLC · Method

Abstract

Dopamine is a neurotransmitter that acts in the nervous system as a chemical messenger, which helps specific neurons to interact with each other. A simple, precise, accurate HPLC method has been developed and validated for determination of Dopamine HCl (DOPHCl) in pharmaceutical formulations. Separation was performed on a C18 column (250 x 4.6 mm, 5 μm) with 50 mM potassium dihydrogen phosphate (pH 2.3) as a mobile phase. Mobile phase flow rate was maintained constant at 1 mL min-1. High sensitivity has been achieved with UV detection at 280 nm. Parameters such as interaction of the active pharmaceutical ingredient and excipients, linearity and repeatability were investigated during analytical method development. Retention time was 6.195 min for DOPHCl. The method has been validated for DOPHCl within the range of 20-100 μg mL-1 (r2=0.9998). This method has shown strong reproducibility and recovery within the determined range. This method can be used successfully in pharmaceutical formulations for routine analysis.

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Bu Makaleye Atıf Yap

AYDIN, B. S., BULDUK, İ. (2020). A Validated HPLC-UV Method for Determination of Dopamine HCl in Injectable Solutions. Eurasian Journal of Biological and Chemical Sciences, Cilt: 3 - Sayı: 2, 116–120. https://ajindex.com/articles/755978

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Kaynakça (8)

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