KaydedilenlerDerginizi ekleyin

Dergiler / Turkish Journal of Medical Sciences / 2013 / Cilt: 43 - Sayı: 2

Demonstration of mutations conferring resistance to lamivudine in liver tissue of chronic hepatitis B patients under lamivudine therapy

İsmail Yaşar AVCI
Can Polat EYİGÜN
Emine GÜNAL
Ayhan KUBAR
Hanefi Cem GÜL
Ömer COŞKUN
Hakan ERDEM
Levent GÖRENEK
Bülent Ahmet BEŞİRBELLİOĞLU
Sayfa
199–202
Yayın Tarihi
2013-02-01
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Therefore, all liver biopsy specimens of patients with severe liver fibrosis should be analyzed using the molecular technique.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:To demonstrate the genotypic lamivudine resistance in liver tissue of patients with negative serum hepatitis B virus (HBV) DNA under long-term lamivudine therapy.
📊
Temel Bulgular & Veri:Materials and methods: Fourteen patients (10 patients with elevated alanine aminotransferase and 4 patients under long-term lamivudine therapy for severe liver disease) with undetected serum HBV DNA were included in the study.
💡
Sonuç & Katkı:Therefore, all liver biopsy specimens of patients with severe liver fibrosis should be analyzed using the molecular technique.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Key words: Mutation · liver tissue · HBV DNA

Özet

To demonstrate the genotypic lamivudine resistance in liver tissue of patients with negative serum hepatitis B virus (HBV) DNA under long-term lamivudine therapy. Materials and methods: Fourteen patients (10 patients with elevated alanine aminotransferase and 4 patients under long-term lamivudine therapy for severe liver disease) with undetected serum HBV DNA were included in the study. A liver biopsy was performed for each patient, and HBV DNA extracted from liver tissue was analyzed with TaqMan real-time polymerase chain reaction. Results: Mutations conferring lamivudine resistance were shown for all 14 patients in their liver tissue-extracted HBV DNA. One patient had rtM204V, 4 patients had rtL180M, 2 had rtL180M + rtM204V, 4 had rtL180M + rtM204I, and 3 had rtL180M + rtM204I + rtM204V substitutions. Conclusion: Analyzing mutation in liver tissues of these patients might help demonstrate resistance earlier and prevent delaying of therapeutic modification. Therefore, all liver biopsy specimens of patients with severe liver fibrosis should be analyzed using the molecular technique.

Abstract

To demonstrate the genotypic lamivudine resistance in liver tissue of patients with negative serum hepatitis B virus (HBV) DNA under long-term lamivudine therapy. Materials and methods: Fourteen patients (10 patients with elevated alanine aminotransferase and 4 patients under long-term lamivudine therapy for severe liver disease) with undetected serum HBV DNA were included in the study. A liver biopsy was performed for each patient, and HBV DNA extracted from liver tissue was analyzed with TaqMan real-time polymerase chain reaction. Results: Mutations conferring lamivudine resistance were shown for all 14 patients in their liver tissue-extracted HBV DNA. One patient had rtM204V, 4 patients had rtL180M, 2 had rtL180M + rtM204V, 4 had rtL180M + rtM204I, and 3 had rtL180M + rtM204I + rtM204V substitutions. Conclusion: Analyzing mutation in liver tissues of these patients might help demonstrate resistance earlier and prevent delaying of therapeutic modification. Therefore, all liver biopsy specimens of patients with severe liver fibrosis should be analyzed using the molecular technique.

🔖

Bu Makaleye Atıf Yap

AVCI, İ. Y., EYİGÜN, C. P., GÜNAL, E., KUBAR, A., GÜL, H. C., COŞKUN, Ö., ERDEM, H., GÖRENEK, L., BEŞİRBELLİOĞLU, B. A. (2013). Demonstration of mutations conferring resistance to lamivudine in liver tissue of chronic hepatitis B patients under lamivudine therapy. Turkish Journal of Medical Sciences, Cilt: 43 - Sayı: 2, 199–202. https://ajindex.com/articles/723489

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (9)

  1. Dikici B, Uzun H, Gözü A, Fidan M. Prevalence of hepatitis B infection among schoolchildren in southeast Turkey. Turk J Med Sci 2009; 39: 289–93. 2. Lavanchy D. Hepatitis B virus epidemiology, disease burden, treatment, and current and emerging prevention and control measures. J Viral Hepat 2004; 11: 97–107. 3. Öner S, Yapıcı G, Şaşmaz CT, Kurt AÖ, Buğdaycı R. Hepatitis B, hepatitis C, HIV, and VDRL seroprevalence of blood donors in Mersin, Turkey. Turk J Med Sci 2011; 41: 335–41. 4. Nwankwo E, Momodu I, Umar I, Musa B, Adeleke S. Seroprevalence of major blood-borne infections among blood Locarnini S. Molecular virology and the development of resistant mutants. Implications for therapy. Semin Liver Dis 2005; 25 (Suppl 1): 9–19.
  2. Allen MI, Deslauriers M, Andrews CW, Tipples GA, Walters KA, Tyrrell DL et al. Identification and characterization of mutations in hepatitis B virus resistant to lamivudine. Lamivudine Clinical Investigation Group. Hepatology 1998; 27: 1670–7.
  3. Chien RN, Yeh CT, Tsai SL, Chu CM, Liaw YF. Determinants for sustained HBeAg response to lamivudine therapy. Hepatology 2003; 38: 1267–73.
  4. Damerow H, Yuen L, Wiegand J, Walker C, Bock CT, Locarnini S et al. Mutation pattern of lamivudine resistance in relation to hepatitis B genotypes: hepatitis B genotypes differ in their lamivudine resistance associated mutation pattern. J Med Virol 2010; 82: 1850–8.
  5. Bozdayi AM, Eyigun CP, Turkyilmaz AR, Avci IY, Pahsa A, Yurdaydin C. A novel pattern (sW195a) in surface gene of HBV DNA due to YSDD (L180M plus M204S) mutation selected during lamivudine therapy and successful treatment with adefovir dipivoxil. J Clin Virol 2004; 31: 76–7.
  6. Zhao P, Wang C, Huang L, Xu D, Li T. Comparison of rescue strategies in lamivudine-resistant patients with chronic hepatitis B. Antiviral Res 2012; 96: 100–4.
  7. Kubar A, Saygun I, Özdemir A, Yapar M, Slots J. Real- time polymerase chain reaction quantification of human cytomegalovirus and Epstein-Barr virus in periodontal pockets and the adjacent gingiva of periodontitis lesions. J Periodont Res 2005; 40: 97–104.
  8. Hunt CM, McGill JM, Allen MI, Condreay LD. Clinical relevance of hepatitis B viral mutations. Hepatology 2000; 31: 1037–44. Zoulim F. Detection of hepatitis B virus resistance to antivirals. J Clin Virol 2001; 21: 243–53. Zoulim F. Assessing hepatitis B virus resistance in vitro and molecular mechanisms of nucleoside resistance. Semin Liver Dis 2002; 22: 23–31.
  9. Thompson AJV, Nguyen T, Iser D, Ayres A, Jackson K, Little