Objective: We investigated the expression of basic Fibroblast Growth Factor (bFGF) and c-kit proto-oncogene in progression to malignancy and métastasés of melanocytic lesions.Methods: Immunohistochemical detection of bFGF and c-kit receptors were performed by peroxidase- antiperoxidase (PAP) technique on compound nevi, vertical growth phase malignant melanoma and metastatic malignant melanoma in 12, 12 and 15 cases respectively.Clinicopathological correlations were analysed by Paerson’s Chi-Square, Student’s T-Test and One Way Anova test.Results: bFGF receptor immunoreactivity rates were 83.3%, 83.3% and 80 % in compound nevi, primary MMs and metastatic MMs respectively, c-kit immunoreactivity was absent in nevus cells, but faintly present in 16.6 % (2 cases) of MM cells.Conclusion: The results of our study suggest that loss of c-kit expression is more important in dermal penetration than métastasés in melanomas.However, bFGF is not shown to be a prognostic factor for MMs.Key Words: c-kit, bFGF, Melanocytic nevi, Malignant melanoma, Metastatic melanoma
📌 3 Maddede Bu Makale
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Amaç & Hipotez:Objective: We investigated the expression of basic Fibroblast Growth Factor (bFGF) and c-kit proto-oncogene in progression to malignancy and métastasés of melanocytic lesions.Methods: Immunohistochemical detection of bFGF and c-kit receptors were performed by peroxidase- antiperoxidase (PAP) technique on compound nevi, vertical growth phase malignant melanoma and metastatic malignant melanoma in 12, 12 and 15 cases respectively.Clinicopathological correlations were analysed by Paerson’s Chi-Square, Student’s T-Test and One Way Anova test.Results: bFGF receptor immunoreactivity rates were 83.3%, 83.3% and 80 % in compound nevi, primary MMs and metastatic MMs respectively, c-kit immunoreactivity was absent in nevus cells, but faintly present in 16.6 % (2 cases) of MM cells.Conclusion: The results of our study suggest that loss of c-kit expression is more important in dermal penetration than métastasés in melanomas.However, bFGF is not shown to be a prognostic factor for MMs.Key Words: c-kit, bFGF, Melanocytic nevi, Malignant melanoma, Metastatic melanoma
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Temel Bulgular & Veri:Objective: We investigated the expression of basic Fibroblast Growth Factor (bFGF) and c-kit proto-oncogene in progression to malignancy and métastasés of melanocytic lesions.Methods: Immunohistochemical detection of bFGF and c-kit receptors were performed by peroxidase- antiperoxidase (PAP) technique on compound nevi, vertical growth phase malignant melanoma and metastatic malignant melanoma in 12, 12 and 15 cases respectively.Clinicopathological correlations were analysed by Paerson’s Chi-Square, Student’s T-Test and One Way Anova test.Results: bFGF receptor immunoreactivity rates were 83.3%, 83.3% and 80 % in compound nevi, primary MMs and metastatic MMs respectively, c-kit immunoreactivity was absent in nevus cells, but faintly present in 16.6 % (2 cases) of MM cells.Conclusion: The results of our study suggest that loss of c-kit expression is more important in dermal penetration than métastasés in melanomas.However, bFGF is not shown to be a prognostic factor for MMs.Key Words: c-kit, bFGF, Melanocytic nevi, Malignant melanoma, Metastatic melanoma
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Sonuç & Katkı:Objective: We investigated the expression of basic Fibroblast Growth Factor (bFGF) and c-kit proto-oncogene in progression to malignancy and métastasés of melanocytic lesions.Methods: Immunohistochemical detection of bFGF and c-kit receptors were performed by peroxidase- antiperoxidase (PAP) technique on compound nevi, vertical growth phase malignant melanoma and metastatic malignant melanoma in 12, 12 and 15 cases respectively.Clinicopathological correlations were analysed by Paerson’s Chi-Square, Student’s T-Test and One Way Anova test.Results: bFGF receptor immunoreactivity rates were 83.3%, 83.3% and 80 % in compound nevi, primary MMs and metastatic MMs respectively, c-kit immunoreactivity was absent in nevus cells, but faintly present in 16.6 % (2 cases) of MM cells.Conclusion: The results of our study suggest that loss of c-kit expression is more important in dermal penetration than métastasés in melanomas.However, bFGF is not shown to be a prognostic factor for MMs.Key Words: c-kit, bFGF, Melanocytic nevi, Malignant melanoma, Metastatic melanoma
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri
Abstract
Objective: We investigated the expression of basic Fibroblast Growth Factor (bFGF) and c-kit proto-oncogene in progression to malignancy and métastasés of melanocytic lesions.Methods: Immunohistochemical detection of bFGF and c-kit receptors were performed by peroxidase- antiperoxidase (PAP) technique on compound nevi, vertical growth phase malignant melanoma and metastatic malignant melanoma in 12, 12 and 15 cases respectively.Clinicopathological correlations were analysed by Paerson’s Chi-Square, Student’s T-Test and One Way Anova test.Results: bFGF receptor immunoreactivity rates were 83.3%, 83.3% and 80 % in compound nevi, primary MMs and metastatic MMs respectively, c-kit immunoreactivity was absent in nevus cells, but faintly present in 16.6 % (2 cases) of MM cells.Conclusion: The results of our study suggest that loss of c-kit expression is more important in dermal penetration than métastasés in melanomas.However, bFGF is not shown to be a prognostic factor for MMs.Key Words: c-kit, bFGF, Melanocytic nevi, Malignant melanoma, Metastatic melanoma
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Bu Makaleye Atıf Yap
KOTİLOĞLU, E., KAYA, H., SEZGİN, S., EKİCİOĞLU, G. (2016). THE ROLE OF BASIC FIBROBLAST GROWTH FACTOR RECEPTOR (bFGF) AND C-KIT RECEPTOR IN PROGRESSION OF CUTANEOUS MELANOCYTIC LESIONS. Marmara Medical Journal, Cilt: 15 - Sayı: 1, 33–38. https://ajindex.com/articles/678411
Alberts B, Bray D, Lewis J, Raff M, Roberts R, Watson JD. Molecular Biology of the Cell. 2nd ed. London: Garland Publishing, Inc. 1989: 681-790. flerlyn M, Mandanti ML, Jambrosic J, Bolen JB, Koprowski ft. Regulatory factors that determine growth and phenotype of normal human melanocytes. Exp Cell Res ¡988; I 79: 322-331. Shih IM, flerlyn M. Autocrine and paracrine roles for growth factors in melanoma. In Vivo 1994; 8:1 13-123. lialaban R, Pan B, Ahn J, Punasaka Y, Gitay- Goren fl, Heufeld G. Growth factors, receptor kinases, and protein tyrosine phosphatases innormal and malignant melonocytes. J Immunother 1992; 12:154-161. Halaban R. Growth factors and tyrosine protein kinases in normal and malignant melanocytes. Cancer Metastasis Rev 1991; 10: 129-140.
Huang S, Luca M, Gutman M, et al. Enforced c- kit expression renders highly metastatic human melanoma cells susceptible to stem cell factor induced apoptosis and inhibits their tumorigenic and metastatic potential. Oncogene 1996; 13: 2339-2347.
Zakut R, Pedis R, Eliyahu S, et al. KIT ligand (mast cell growth factor) inhibits the growth of KIT-expressing melanoma cells. Oncogene 1993; 8: 2221-2229.
Tsuura Y, Hiraki H, Watanabe K, et af Preferential localization of c-kit product in tissue mast cells, basal cells of skin, epithelial cells of breast, small cell lung carcinoma and seminoma/dysgerminoma in human: immunohistochemical study on formalin- fixed- paraffin-embedded tissues. Virchows Arch 1994; 424: 135-141.
Al-Alousi S, Barnhill R, Blessing K, Barksdale S. The prognostic significance of basic fibroblast growth factor in cutaneous malignant melanoma. J Cutan Pathol 1996; 23: 506-510.
Luo D, Chen H, Searles Cj, Jumbow K. Coordinated mRHA expression of c-Kit with tyrosinase and TRP-1 in melanine pigmentation of normal and malignant human melanocytes and transient activation of tyrosinase by Kit/SCP-R. Melanoma Res 1995; 5: 303-309. I I. Hatali PG, nicotra MR, Winkler AB, Cavaliere R, Bigotti A, Ullrich A. Progression of human cutaneous melanoma is associated with loss of expression of c-kit proto-oncogene receptor. Int J Cancer 1992; 52: 197-201.
Yarden Y, Kuang WJ, Yang-Peng T, et af Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J 1988; 7:1003- 1011. Giebel LB, Strunk KM, Holmes 5/1, Spritz RA. Organization and nucleotide sequence of the human KIT (mast/stem cell growth factor receptor) proto-oncogene. Oncogene 1992;7:2207-221 7. Hiroto S, Isoz