Yapay Zeka Hızlı Bakış & Çıkarımlar
AI InsightThe outgrowth (cultivated limbal epithelial cells) is then surgicallytransferred to the recipient eye.Due to changing regulations concerning cell-based therapy, the implementation of cultivated limbal epithelial transplantation inaccordance with Good Laboratory Practice using xenobiotic-free systems is becoming widely accepted both in Turkey and worldwide.
📌 3 Maddede Bu Makale
Abstract
The cornea is the outermost tissue of the eye and it must be transparent for the maintenance of good visual function. The superficialepithelium of the cornea, which is renewed continuously by corneal stem cells, plays a critical role in the permanence of this transparency.These stem cells are localized at the cornea-conjunctival transition zone, referred to as the limbus. When this zone is affected/destroyed,limbal stem cell deficiency ensues. Loss of limbal stem cell function allows colonization of the corneal surface by conjunctival epithelium.Over 6 million people worldwide are affected by corneal blindness, and limbal stem cell deficiency is one of the main causes. Fortunately,it is becoming possible to recover vision by autologous transplantation of limbal cells obtained from the contralateral eye in unilateralcases. Due to the potential risks to the donor eye, only a small amount of tissue can be obtained, in which only 1-2% of the limbalepithelial cells are actually limbal stem cells. Vigorous attempts are being made to expand limbal stem cells in culture to preserve oreven enrich the stem cell population. Ex vivo expanded limbal stem cell treatment in limbal stem cell deficiency was first reported in1997. In the 20 years since, various protocols have been developed for the cultivation of limbal epithelial cells. It is still not clear whichmethod promotes effective stem cell viability and this remains a subject of ongoing research. The most preferred technique for limbalcell culture is the explant culture model. In this approach, a small donor eye limbal biopsy is placed as an explant onto a biocompatiblesubstrate (preferably human amniotic membrane) for expansion. The outgrowth (cultivated limbal epithelial cells) is then surgicallytransferred to the recipient eye.Due to changing regulations concerning cell-based therapy, the implementation of cultivated limbal epithelial transplantation inaccordance with Good Laboratory Practice using xenobiotic-free systems is becoming widely accepted both in Turkey and worldwide.