Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses

Amaç & Hipotez:Objectives: To compare biofilm formations of two Staphylococcus epidermidis (S. epidermidis) isolates with known biofilm formationcapacities on four different intraocular lenses (IOL) that have not been studied before.Materials and Methods: Two isolates obtained from ocular surfaces and identified in previous studies and stored at -86 °C in 15%glycerol in the microbiology laboratory of the Anadolu University Department of Biology were purified and used in the study.

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Temel Bulgular & Veri:The isolateswere S. epidermidis KA 15.8 (ICA+), a known biofilm producer isolate positive for icaA, icaD and bap genes, and S. epidermidis KA 14.5 (ICA-),known as a non-biofilm producer isolate negative for icaA, icaD and bap genes.

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Sonuç & Katkı:The highest biofilm production was obtained on IOL C (PMMA) (p

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Alan Parametreleri

Abstract

Objectives: To compare biofilm formations of two Staphylococcus epidermidis (S. epidermidis) isolates with known biofilm formationcapacities on four different intraocular lenses (IOL) that have not been studied before.Materials and Methods: Two isolates obtained from ocular surfaces and identified in previous studies and stored at -86 °C in 15%glycerol in the microbiology laboratory of the Anadolu University Department of Biology were purified and used in the study. The isolateswere S. epidermidis KA 15.8 (ICA+), a known biofilm producer isolate positive for icaA, icaD and bap genes, and S. epidermidis KA 14.5 (ICA-),known as a non-biofilm producer isolate negative for icaA, icaD and bap genes. The biofilm formation capacities of the 2 isolates on 4 differentIOLs were compared. Two of the IOLs were acrylic (UD613 [IOL A], Turkey; SA60AT [IOL B], USA), and the other two were polymethylmethacrylate (PMMA) (B60130C [IOL C], India; B55125C [IOL D], India). Bacterial enumeration and optical density measurements weredone from biofilms that formed on the IOLs. Biofilms were imaged using scanning electron microscopy.Results: Mean bacterial counts on the IOLs were 7.1±0.4 log10 CFU/mL with the ICA+ isolate, and 6.7±0.8 log10 CFU/mL with theICA- isolate; there were no statistically significant differences. Biofilm formation was lower with acrylic lenses than PMMA lenses withboth isolates (p=0.009 and p=0.013). The highest biofilm production was obtained on IOL C (PMMA) (p

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Bu Makaleye Atıf Yap

ÖZMEN, A. T., KIVANÇ, M., KIVANÇ, S. A., KILIÇ, V., GÜLLÜLÜ, G. (2022). Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses. Türk Oftalmoloji Dergisi, Cilt: 47 - Sayı: 2, 68–73. https://ajindex.com/articles/637842

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Kaynakça (12)

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