Yapay Zeka Hızlı Bakış & Çıkarımlar
AI InsightTissue-specific expression ofrcTRαandrcTRβsuggests thatthyroid hormones play various roles in different organs or tissues at different developmental stages of metamorphosis.
📌 3 Maddede Bu Makale
Abstract
To explore the role of thyroid hormones in organ remodeling during the metamorphosis of amphibians, the cDNAs of twothyroid hormone receptors ( TRαandTRβ ) inRana chensinensiswere identified. The 1257 bp ofrcTRαcDNA encoding 418 amino acidsand the 1122 bp ofrcTRβcDNA encoding 373 amino acids were cloned. Their polypeptide sequences contain two highly conservedcysteine-rich zinc fingers in the DNA-binding domain, while rcTRβ is 42 amino acids shorter in its A/B domain when compared withrcTRα. Fifty-five amino acids differed between the TRα and TRβ domains. ThercTRαandrcTRβmRNA levels in the liver, brain, skin,and tail of tadpoles were analyzed via qRT-PCR during metamorphosis. The results showed that the changes ofrcTRαmRNA levelswere moderate while those ofrcTRβwere dramatic in all 4 tissues during metamorphosis. In addition, the levels ofrcTRβmRNA wererelatively low at premetamorphosis and reached a peak around the climax. Tissue-specific expression ofrcTRαandrcTRβsuggests thatthyroid hormones play various roles in different organs or tissues at different developmental stages of metamorphosis.