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Dergiler / Turkish Journal of Zoology / 2019 / Cilt: 43 - Sayı: 2

Earthworm, a novel in vivo system to validate antimitotic compounds

Kamaraj RAJAMANIKKAM
Sudhakar SIVASUBRAMANIAM
Ganapathy NADANA RAJAVADIVU
Senthamarai Kannan BALAJI
Karuppaiah PALANICHELVAM
Subramanian ELAIYA RAJA
Sayfa
153–163
Yayın Tarihi
2022-11-04
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

It is simple, reproducible, and cost-efficient compared to animal cell line-based methods.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:We report here that earthworm can be used as an in vivo system to prescreen antimitotic compounds.
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Temel Bulgular & Veri:The antimitoticactivity of colchicine and the rhizome extract was confirmed by methods with Allium cepa root tip, earthworm regeneration, andMTT (3-(4,5-dimethyl thiazolyl-2-yl)-2,5-diphenyltetrazolium bromide).
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Sonuç & Katkı:It is simple, reproducible, and cost-efficient compared to animal cell line-based methods.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

We report here that earthworm can be used as an in vivo system to prescreen antimitotic compounds. The known antimitoticcompound colchicine and the aqueous extract of Acorus calamus L. rhizome were used to examine this system. The antimitoticactivity of colchicine and the rhizome extract was confirmed by methods with Allium cepa root tip, earthworm regeneration, andMTT (3-(4,5-dimethyl thiazolyl-2-yl)-2,5-diphenyltetrazolium bromide). An earthworm regeneration assay examined the regenerationability of tissues from amputated regions of the earthworm. All 3 assays showed that cell division is inhibited with colchicine and theaqueous extract of A. calamus rhizome. Histology studies with amputated earthworms confirmed that the development of the blastemawas observed only in control worms. Immunohistochemistry analysis with proliferation cell nuclear antigen and phospho serine 10histone H3 antibodies showed that amputated worms treated with colchicine or the aqueous extract of A. calamus did not have activecell division. MTT assay with the MCF-7 cell line (human breast carcinoma) further confirmed that the aqueous extract of rhizomeaffected cell proliferation. Our results suggest that the earthworm could be used to prescreen the antimitotic potential of plant extractsor other unknown compounds. It is simple, reproducible, and cost-efficient compared to animal cell line-based methods.

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Bu Makaleye Atıf Yap

RAJAMANIKKAM, K., SIVASUBRAMANIAM, S., RAJAVADIVU, G. N., BALAJI, S. K., PALANICHELVAM, K., RAJA, S. E. (2022). Earthworm, a novel in vivo system to validate antimitotic compounds. Turkish Journal of Zoology, Cilt: 43 - Sayı: 2, 153–163. https://ajindex.com/articles/616015

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Kaynakça (12)

  1. Baldo BA, Pham NH (2013). Drug Allergy: Clinical Aspects, Diagnosis, Mechanisms, Structure–Activity Relationships. New York, NY, USA: Springer. Bhattacharyya B, Panda D, Gupta S, Banerjee M (2008). Anti‐mitotic activity of colchicine and the structural basis for its interaction with tubulin. Med Res Rev 28: 155-183.
  2. Cella D, Peterman A, Hudgens S, Webster K, Socinski MA (2003). Measuring the side effects of taxane therapy in oncology. Cancer 98: 822-831.
  3. Coker PS, Radecke J, Guy C, Camper ND (2003). Potato disc tumor induction assay: a multiple mode of drug action assay. Phytomedicine 10: 133-138.
  4. Denizot F, Lang R (1986). Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 89: 271-277.
  5. Doke SK, Dhawale SC (2015). Alternatives to animal testing: a review. Saudi Pharm J 23: 223-229.
  6. Eigsti OJ (1939). A cytological study of colchicine effects in the induction of polyploidy in plants. Protoplasma 32: 318-318.
  7. Fadeyi SA, Fadeyi OO, Adejumo AA, Okoro C, Myles El (2013). In vitro anticancer screening of 24 locally used Nigerian medicinal plants. BMC Complement Altern Med 13: 79. Festing S, Wilkinson R (2007). The ethics of animal research: talking point on the use of animals in scientific research. EMBO Rep 8: 526-530.
  8. Foley JF, Dietrich DR, Swenberg JA Maronpot RR (1991). Detection and evaluation of proliferating cell nuclear antigen (PCNA) in rat tissue by an improved immunohistochemical procedure. J Histotechnol 14: 237-241. Fouché G, Cragg GM, Pillay P, Kolesnikova N, Maharaj VJ Senabe J (2008). In vitro anticancer screening of South African plants. J Ethnopharmacol 119: 455-461.
  9. Gascoigne KE, Taylor SS (2009). How do anti-mitotic drugs kill cancer cells? J Cell Sci 122: 2579-2585.
  10. Imam H, Riaz Z, Azhar M, Sofi G, Hussain A (2013). Sweet flag (Acorus calamus Linn.): an incredible medicinal herb. Int J Green Pharm 7: 288. Jordan MA, Wilson L (2004). Microtubules as a target for anticancer drugs. Nat Rev Cancer 4: 253. Kalidas RM, Raja SE, Mydeen SAKNM, Samuel SCJR, Durairaj SCJ, Nino GD, Palanichelvam K, Vaithi A, Sudhakar S (2015). Conserved lamin A protein expression in differentiated cells in the earthworm Eudrilus eugeniae. Cell Biol Int 39: 1036-1043.
  11. Khazir J, Mir BA, Pilcher L, Riley DL (2014). Role of plants in anticancer drug discovery. Phytochem Lett 7: 173-181.
  12. Kundu LM, Ray S (2017). Mitotic abnormalities and micronuclei inducing potent