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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2019 / Cilt: 43 - Sayı: 5

Phylogenetic analyses of P32, RPO30, GPCR, ORF117, and Kelch-like genes of Tunisian sheeppox virus isolates

Jihene LACHHEB
Houssem MASTOUR
Aicha TURKI
Jihene NSIRI
Abdeljelil GHRAM
Imen LARBI
Imen EL BEHI
Sayfa
603–614
Yayın Tarihi
2022-11-04
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Our study proved the efficiency of using these five genes for the identification and differentiation of capripoxvirus species, andthe GPCR gene is recommended for phylogenetic studies.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Sheeppox is an economically important disease of small ruminants caused by the sheeppox virus (SPPV).
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Temel Bulgular & Veri:Eleven sheeppox virus isolates from five different regions of Tunisia were collectedbetween 2010 and 2016.
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Sonuç & Katkı:Our study proved the efficiency of using these five genes for the identification and differentiation of capripoxvirus species, andthe GPCR gene is recommended for phylogenetic studies.
🔬Tür:Araştırma Makalesi
📍Kapsam:2010 Verileri
⚙️Değişkenler:Alan Parametreleri

Abstract

Sheeppox is an economically important disease of small ruminants caused by the sheeppox virus (SPPV). Sheeppoxvirus (SPPV), goatpox virus, and lumpy skin disease virus are capripoxviruses, antigenically related but genetically distinct. Theirdifferentiation requires analysis at a molecular level. Eleven sheeppox virus isolates from five different regions of Tunisia were collectedbetween 2010 and 2016. SPPV was detected by real-time PCR using SYBR Green technology. Primers amplifying the entire Kelch-likegene were designed. Then the GPCR, RPO30, P32, ORF117, and Kelch-like genes were molecularly analyzed to show the different specificlineage patterns of each species. Phylogenetic analyses revealed three monophyletic and distinct specific lineage clusters as per their hostorigins. Our study proved the efficiency of using these five genes for the identification and differentiation of capripoxvirus species, andthe GPCR gene is recommended for phylogenetic studies.

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Bu Makaleye Atıf Yap

LACHHEB, J., MASTOUR, H., TURKI, A., NSIRI, J., GHRAM, A., LARBI, I., BEHI, I. E. (2022). Phylogenetic analyses of P32, RPO30, GPCR, ORF117, and Kelch-like genes of Tunisian sheeppox virus isolates. Turkish Journal of Veterinary and Animal Sciences, Cilt: 43 - Sayı: 5, 603–614. https://ajindex.com/articles/615175

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Kaynakça (14)

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