Yapay Zeka Hızlı Bakış & Çıkarımlar
AI InsightThese results indicate that theuse of PCR in the routine analysis of meat preparations for the presence ofSarcocystisspecies should be increased, since the applicationof this technique would provide analysts with more conclusive results than examination by microscope.
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Abstract
Sarcocystisspecies are the most commonly encountered parasitic organisms in animals.Sarcocystis hominis( Sarcocystisbovihominis ), the species found in beef cattle, is not visible macroscopically in muscle tissue. In recent years it has been establishedthat, in addition to being a zoonotic parasite transmitted via the consumption of beef, the parasite produces toxins and causes foodpoisoning. Therefore, this study was undertaken in order to determine the extent to whichSarcocystisspecies are present in mincedmeat, meatballs, and fermented sausages produced from beef and marketed in Kars. A total of 150 samples, 50 of each product, werecollected in the city center from butcher s shops, from businesses that produce such items, and from general stores. In order to detectsarcocysts in the samples, the cysts revealed by an enzymatic digestion test were examined microscopically. Genomic DNA extractedfrom the samples was stored at20 °C for analysis by polymerase chain reaction (PCR). The microscopic analysis revealed that theparasite was present in 2 samples of minced meat (4%) and 1 sample of meatballs (2%). Analysis by PCR revealed that the parasite waspresent in 14 samples of minced meat (28%), 34 samples of meatballs (68%), and 1 sample of sausage (2%). These results indicate that theuse of PCR in the routine analysis of meat preparations for the presence ofSarcocystisspecies should be increased, since the applicationof this technique would provide analysts with more conclusive results than examination by microscope.