KaydedilenlerDerginizi ekleyin

Dergiler / Turkish Journal of Medical Sciences / 2005 / Cilt: 35 - Sayı: 6

Development of ELISA systems for measurement of human tumor necrosis factor-alpha (TNF-alpha )

Cemalettin AYBAY
Ayşegül YÜCEL
Sayfa
385–393
Yayın Tarihi
2022-11-04
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Two different types of ELISA systems with high sensitivity and specificity were developed to measure hTNF-alpha level both in human serum and cell culture supernatant.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:The aim of this study was to develop a human tumor necrosis factor alpha (hTNF-alpha) ELISA system because specific and sensitive measurement of low levels of circulating TNF-alpha is very important for enlightening the immunopathologial mechanisms associated with TNF-alpha.
📊
Temel Bulgular & Veri:Monoclonal antibodies 6A4c and 8A6 were produced against hTNF-alpha and were used as the capture antibody and the tracer antibody respectively for the first hTNF-alpha ELISA system (6A4c/biotin-8A6).
💡
Sonuç & Katkı:Two different types of ELISA systems with high sensitivity and specificity were developed to measure hTNF-alpha level both in human serum and cell culture supernatant.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

The aim of this study was to develop a human tumor necrosis factor alpha (hTNF-alpha) ELISA system because specific and sensitive measurement of low levels of circulating TNF-alpha is very important for enlightening the immunopathologial mechanisms associated with TNF-alpha. Monoclonal antibodies 6A4c and 8A6 were produced against hTNF-alpha and were used as the capture antibody and the tracer antibody respectively for the first hTNF-alpha ELISA system (6A4c/biotin-8A6). Murine polyclonal IgG was used as the tracer antibody in the second hTNF-alpha ELISA system (6A4c/biotin-polyclonal IgG). Both systems could detect both recombinant hTNF-alpha and native hTNF-alpha. The detection limits defined as minimal concentration of hTNF-alpha were less than 4 pg/ml for the first and less than 12 pg/ml for the second ELISA systems. 6A4c/biotin-8A6 system resulted in some non-specific reactions to some extent with human sera; however, 6A4c/biotin-polyclonal IgG system produced acceptable background levels with human sera. A prominent inhibitory effect of TNF receptors-I and -II did not occur in any of the ELISA systems at physiological concentrations. Two different types of ELISA systems with high sensitivity and specificity were developed to measure hTNF-alpha level both in human serum and cell culture supernatant.

🔖

Bu Makaleye Atıf Yap

AYBAY, C., YÜCEL, A. (2022). Development of ELISA systems for measurement of human tumor necrosis factor-alpha (TNF-alpha ). Turkish Journal of Medical Sciences, Cilt: 35 - Sayı: 6, 385–393. https://ajindex.com/articles/611992

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (14)

  1. Bharat B, Aggarwal B, Samanta A et al. TNF-oc. The TNF Family. Cytokine Reference, Volume 1, (Eds-in-chief: Oppenheim JJ, Feldman M. Eds: Durum SK, Hirano T, Vilcek J, Nicola NA), Academic Press-A Hartcout Science and Technology Company, London, 2001, pp 413-434
  2. Aggarwal BB, Reddy S. Tumor necrosis factor (TNF). Guidebook to Cytokines and Their Receptors. (Ed. Nicola NA), Sambrook & Tooze Publication-Oxford University Press, Oxfrod, 1994, pp 103-104
  3. Beutler B. Tumor necrosis factors: the molecules and their emerging roles in medicine, Raven Publishing, New York, 1992, pp 1-590
  4. Aybay C, Imir T. Development of a rapid, single-step procedure using protein G affinity chromatography to deplete fetal calf serum of its IgG and to isolate murine IgGl monoclonal antibodies from supernatants of hybridoma cells. J Immunol Methods 233: 77-81,2000
  5. Wallach D. TNF Ligand and TNF/NGF Receptor Families. The TNF Family. Cytokine Reference, Volume 1, (Eds-in-chief: Oppenheim JJ, Feldman M. Eds: Durum SK, Hirano T, Vilcek J, Nicola NA), Academic Press-A Hartcout Science and Technology Company, London, 2001, pp 377-412
  6. Aggarwal BB, Natarajan K. Tumor necrosis factors. Developments during last decade. Eur Cytokine Network 7: 93-124, 1996
  7. Tracey KJ, Ceramy A. Tumor necrosis factor, other cytokines and disease. Ann. Rev Cell Biol 9: 317-343, 1993
  8. Gardlung B, Sjolin J, Nilson A et al. Plasma levels of cytokines in primary septic shock in humans: Correlation with disease severity. J Infect Dis 172: 296-301, 1995
  9. Mackay F, Railed SL. TNF Ligands and receptors in autoimmunity:. an update. Curr Opin Immunol. 14: 783-790, 2002
  10. Klausner JD, Freedman VH, Kaplan G. Thalidomide as an anti-TNFa inhibitor: implications for clinical use (review). Clin Immunol Immunopathol 81: 219-223, 1996
  11. Walsh CJ, Sugerman HJ, Mullen PG et al. Monoclonal antibody to tumor necrosis factor alpha attenuates cardiopulmonary dysfunction in porcine gram-negative sepsis. Arch Surg 127:138-145, 1992
  12. Williams RO, Feldmann M, Maini RN. Anti-tumor necrosis factor ameliorates joint disease in murine collagen-induced arthritis.Proc Natl Acad Sci USA 89: 9784-9788, 1992
  13. Neutrah M, Fuss I, Pasparakis M, et al. Predominant pathogenic role of tumor necrosis factor in experimental colitis in mice. Eur J Immunol 27: 1743-1750, 1997
  14. Baker D, Butler D, Scallon BJ et al. Control of established experimental allergic encephalomyelitis by inhibition of tumor necrosis fact