KaydedilenlerDerginizi ekleyin

Dergiler / Turkish Journal of Medical Sciences / 2008 / Cilt: 38 - Sayı: 6

Peripheral Blood Lymphocyte Activation and RANTES Levels in Asthma

Bilun GEMİCİOĞLU
Gaye ERTEN
Bülent TUTLUOĞLU
Benan MÜSELLİM
Günnur DENİZ
Sayfa
531–536
Yayın Tarihi
2022-11-05
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Conclusions: These changes in the activation state of T-cells suggest an active role of T lymphocytes in the pathogenesis of atopic and non-atopic asthma.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Aim: Bronchial mucosal inflammation is one of the major characteristics of atopic asthma.
📊
Temel Bulgular & Veri:Th2 activation and the related cytokine profile, eosinophil activation and infiltration play the main role in the pathogenesis of atopic asthma.
💡
Sonuç & Katkı:Conclusions: These changes in the activation state of T-cells suggest an active role of T lymphocytes in the pathogenesis of atopic and non-atopic asthma.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Asthma · lymphocyte activation · RANTES

Özet

Aim: Bronchial mucosal inflammation is one of the major characteristics of atopic asthma. Th2 activation and the related cytokine profile, eosinophil activation and infiltration play the main role in the pathogenesis of atopic asthma. The aim of this study was to demonstrate the activation and RANTES (regulated on activation, normal T-cell expressed and presumably secreted) expression of peripheral blood lymphocytes of non-atopic and atopic asthmatic patients. Materials and Methods: CD3, CD4, CD8, CD16, CD23, CD25, CD45RA and CD45RO expressions were determined in 22 asthma patients and 20 healthy control subjects by flow cytometry, and RANTES levels were measured by ELISA. Statistical analysis was performed by using Student´s t and Mann-Whitney U test. Results: CD45RO and CD23 expressions were significantly higher in asthma patients compared to control subjects (P = 0.009 and P = 0.004, respectively), and similarly, an increase in CD25 expression was also shown in asthmatics (P = 0.004). However, there was no difference in RANTES secretion of peripheral blood lymphocytes in asthmatics compared to the control group (P = 0.08). Atopic and non-atopic asthmatics (13 vs. 9) were compared, and atopic asthmatics showed significant increase in CD25 and CD23 expressions (P = 0.009 and P = 0.02, respectively). Conclusions: These changes in the activation state of T-cells suggest an active role of T lymphocytes in the pathogenesis of atopic and non-atopic asthma.

Abstract

Aim: Bronchial mucosal inflammation is one of the major characteristics of atopic asthma. Th2 activation and the related cytokine profile, eosinophil activation and infiltration play the main role in the pathogenesis of atopic asthma. The aim of this study was to demonstrate the activation and RANTES (regulated on activation, normal T-cell expressed and presumably secreted) expression of peripheral blood lymphocytes of non-atopic and atopic asthmatic patients. Materials and Methods: CD3, CD4, CD8, CD16, CD23, CD25, CD45RA and CD45RO expressions were determined in 22 asthma patients and 20 healthy control subjects by flow cytometry, and RANTES levels were measured by ELISA. Statistical analysis was performed by using Student´s t and Mann-Whitney U test. Results: CD45RO and CD23 expressions were significantly higher in asthma patients compared to control subjects (P = 0.009 and P = 0.004, respectively), and similarly, an increase in CD25 expression was also shown in asthmatics (P = 0.004). However, there was no difference in RANTES secretion of peripheral blood lymphocytes in asthmatics compared to the control group (P = 0.08). Atopic and non-atopic asthmatics (13 vs. 9) were compared, and atopic asthmatics showed significant increase in CD25 and CD23 expressions (P = 0.009 and P = 0.02, respectively). Conclusions: These changes in the activation state of T-cells suggest an active role of T lymphocytes in the pathogenesis of atopic and non-atopic asthma.

🔖

Bu Makaleye Atıf Yap

GEMİCİOĞLU, B., ERTEN, G., TUTLUOĞLU, B., MÜSELLİM, B., DENİZ, G. (2022). Peripheral Blood Lymphocyte Activation and RANTES Levels in Asthma. Turkish Journal of Medical Sciences, Cilt: 38 - Sayı: 6, 531–536. https://ajindex.com/articles/611770

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (13)

  1. Corrigan CJ, Hartnell A, Kay AB. T lymphocyte activation in acute severe asthma. Lancet 1988; 21(1): 1129-32.
  2. Wilson JW, Djukanovic R, Howarth PH, Holgate ST. Lymphocyte activation in BAL and peripheral blood in atopic asthma. Am Rev Resp Dis 1992; 145: 958-60.
  3. Djukanovic R, Roche WR, Wilson JW, Beasley CR, Twentyman OP, Howarth RH et al. Mucosal inflammation in asthma. Am Rev Resp Dis 1990; 142(2): 434-57.
  4. Gratziou C, Caroll M, Wals A, Howarth PH, Holgate ST. Early changes in T lymphocytes recovered by BAL after local challenge of asthmatic airways. Am Rev Resp Dis 1992; 145: 1259-64.
  5. Jung C, Prinz JC, Rieber EP, Ring J. A reduction in allergen- induced Fc epsilon R2/CD23 expression on peripheral B cells correlates with successful hyposensitization in grass pollinosis. J Allergy Clin Immunol 1995; 95: 77-87.
  6. Poryadin GV, Zhuravleva NE, Salmasi JM, Kazimirsky AN, Semenova LY, Polner SA et al. Immunological mechanisms of recovery from an acute stage in patients with atopic bronchial asthma. Russ J Immunol 2002; 7(3): 259-64.
  7. Bonnefoy J, Guchet JF, Life P, Graber J, Aubry P, Leconet S. Regulation of IgE synthesis by CD23/CD21 interactions. Int Arch Allergy Immunol 1995; 107: 40-2.
  8. Maliszewski CR, Grabstein K, Fanslow WC, Armitage R, Spriggs MK, Sato TA. Recombinant CD40 ligand stimulation of murine B cell growth and differentiation: cooperative effects of cytokines. Eur J Immunol 1993; 23: 1044-9.
  9. Cernadas M, De Sanctis GT, Krinzman SJ, Mark D, Donovan CE, Listman JA et al. CD23 and allergic pulmonary inflammation: potential role as an inhibitor. Am J Respir Cell Mol Biol 1999; 20: 1-8.
  10. Beverly PCL, Merkenschlager M, Terry L. Phenotypic diversity of the CD45 antigen and its relationship to function. Immunol 1988; 88: 3-5.
  11. Poston RN, Chanez P, Lacoste JY, Lichfield T, Lee TH, Bousquet J. Immunohistochemical characterization of the cellular infiltration in asthmatic bronchi. Am Rev Resp Dis 1992; 145: 918-21.
  12. Bradley BL, Azzawi M, Jacobson M, Assoufi B, Collins JW, Irani AM et al. Eosinophils, T lymphocytes, mast cells, neutrophils and macrophages in bronchial biopsy specimens from atopic subjects with asthma: comparison with biopsy specimens from atopic subjects without asthma and normal control subjects and relationship to bronchial hyperresponsiveness. J Allergy Clin Immunol 1991; 88: 661-74. Leung DYM. Molecular basis of allergic disease. Mol Genet Metab 1998; 63: 157-67.
  13. John M, Oltmanns U, Binder C, Meiners S, Gellert K, Chung KF et al. Inhibition of che