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Dergiler / Turkish Journal of Medical Sciences / 2017 / Cilt: 47 - Sayı: 1

Dysregulation of the DKK1 gene in pediatric B-cell acute lymphoblastic leukemia

Özden HATIRNAZ NG
Müge SAYİTOĞLU
Sinem FIRTINA
Yücel ERBİLGİN
Uğur ÖZBEK
Sayfa
357–363
Yayın Tarihi
2022-11-05
DOI
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Conclusion: DKK1 methylation exists in some of cases but is not sufficient for WNT pathway activation alone in pediatric B-ALL.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Background/aim: The canonical Wingless-type (WNT) pathway is involved in normal hematopoietic cell development and deregulated WNT signaling is implicated in the development of hematological malignancies.
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Temel Bulgular & Veri:Dickkopf 1 (DKK1) acts as a modulator of the β-catenin regulated canonical pathway.
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Sonuç & Katkı:Conclusion: DKK1 methylation exists in some of cases but is not sufficient for WNT pathway activation alone in pediatric B-ALL.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

Background/aim: The canonical Wingless-type (WNT) pathway is involved in normal hematopoietic cell development and deregulated WNT signaling is implicated in the development of hematological malignancies. Dickkopf 1 (DKK1) acts as a modulator of the β-catenin regulated canonical pathway. Activation of DKK1 leads to apoptosis and growth suppression, whereas silencing by promoter hypermethylation results in abnormal WNT activation. The secreted inhibitor Dickkopf can antagonize WNT signaling by competitively binding to low density lipoprotein receptors (LRPs) 5 and 6. Materials and methods: We studied DKK1 gene promoter methylation and investigated DKK1, β-catenin, LRP5, and LRP6 mRNA levels in B-cell acute lymphoblastic leukemia (B-ALL) patients (n = 90). Methylation-specific PCR and bisulfite sequencing were used for methylation profiling and quantitative real-time PCR was used for mRNA detection. Results: The DKK1 gene was examined for its promoter methylation and only 33% of patients were found methylated. On the other hand, B-ALL cases showed high expression of DKK1 (P = 0.01), LRP5 (P = 0.04), and LRP6 (P = 0.02) compared to normal bone marrow cells. Conclusion: DKK1 methylation exists in some of cases but is not sufficient for WNT pathway activation alone in pediatric B-ALL.

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Bu Makaleye Atıf Yap

NG, Ö. H., SAYİTOĞLU, M., FIRTINA, S., ERBİLGİN, Y., ÖZBEK, U. (2022). Dysregulation of the DKK1 gene in pediatric B-cell acute lymphoblastic leukemia. Turkish Journal of Medical Sciences, Cilt: 47 - Sayı: 1, 357–363. https://ajindex.com/articles/610586

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Kaynakça (12)

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