Yapay Zeka Hızlı Bakış & Çıkarımlar
AI InsightThedesirable bands were observed in clinical samples with high accuracy.Conclusion: It should be pointed out that using a precisely designed primer pair would bring about early infection detection, moresuccess to detect all natural variants and higher cost-to-efficacy ratio in comparison to other detection methods.
📌 3 Maddede Bu Makale
Abstract
Background: Conventional methods of detecting Brucella spp. suffer from technical and biological complications. Besides, newlycharacterized species of the genus Brucella could be neglected by previously designed polymerase chain reaction (PCR) tests. Therefore,a more accurate PCR-based test seems to be imminently needed.Materials and methods: Blood samples were collected from 39 patients diagnosed with brucellosis and 25 healthy controls. Multiplesequence alignments (MSA) were performed on 500 Omp2-related protein and gene sequences. Thereafter, specific primers weredesigned and synthesized for the regions with highest conservancy. The collected samples were assessed by PCR test. To overcome thecross-reactivity issue, PCR thermal program was optimized regarding annealing time and temperature.Results: The MSA results indicated that the N terminus region of the Omp2 protein (DNA 5’ end) is associated with highest conservancy.Primers with highest specificity were designed and synthesized. A two-step PCR reaction was successfully designed and optimized. Thedesirable bands were observed in clinical samples with high accuracy.Conclusion: It should be pointed out that using a precisely designed primer pair would bring about early infection detection, moresuccess to detect all natural variants and higher cost-to-efficacy ratio in comparison to other detection methods.