This is the first record for a LFY-like gene, TtLFY, expressed in the shoot tips of an underground plant section and for an AP2-like gene transcript found in all tissues, similar to a housekeeping gene.
📌 3 Maddede Bu Makale
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Amaç & Hipotez:LEAFY (LFY), APETALA1 (AP1), and APETALA2 (AP2) genes encode three different transcription factors that control and regulate flower initiation and development in Arabidopsis.
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Temel Bulgular & Veri:By using 3 - and 5 -RACE analysis, we isolated and sequentially characterized TtLFY (a LEAFY-like gene), TtAP1 (a MADS-box like gene), and TtAP2 (an AP2/ERBF-like gene) in Thermopsis turcica, an unusual endemic legume species with three free carpellated flower structure.
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Sonuç & Katkı:This is the first record for a LFY-like gene, TtLFY, expressed in the shoot tips of an underground plant section and for an AP2-like gene transcript found in all tissues, similar to a housekeeping gene.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri
Abstract
LEAFY (LFY), APETALA1 (AP1), and APETALA2 (AP2) genes encode three different transcription factors that control and regulate flower initiation and development in Arabidopsis. By using 3 - and 5 -RACE analysis, we isolated and sequentially characterized TtLFY (a LEAFY-like gene), TtAP1 (a MADS-box like gene), and TtAP2 (an AP2/ERBF-like gene) in Thermopsis turcica, an unusual endemic legume species with three free carpellated flower structure. Semiquantitative RT-PCR analysis for 18 different vegetative and reproductive tissues of T. turcica indicated TtLFY transcripts mainly in the shoot tips and young floral buds and TtAP1 transcripts in the sepals and petals; however, TtAP2 transcripts were detected in all tissues. This is the first record for a LFY-like gene, TtLFY, expressed in the shoot tips of an underground plant section and for an AP2-like gene transcript found in all tissues, similar to a housekeeping gene.
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Bu Makaleye Atıf Yap
KARAKURT, Y., CENKCİ, S., DEDEOĞLU, A., KAHRAMAN, B., KARGIOĞLU, M. (2022). Isolation and expression analysis of three different flowering genes(TtLFY, TtAP1, and TtAP2) from an unusual legume species, Thermopsis turcica. Turkish Journal of Botany, Cilt: 40 - Sayı: 5, 447–460. https://ajindex.com/articles/601057
Aukerman MJ, Sakai H (2003). Regulation of flowering time and floral organ identity by a microRNA and its APETALA2-like target genes. The Plant Cell Online 15: 2730-2741.
Ballerini ES, Kramer EM (2011). Environmental and molecular analysis of the floral transition in the lower eudicot Aquilegia formosa. EvoDevo 2: 1-20. Benlloch R, dErfurth I, Ferrandiz C, Cosson V, Beltrán JP, Cañas LA, Ratet P (2006). Isolation of mtpim proves Tnt 1 a useful reverse genetics tool in Medicago truncatula and uncovers new aspects of AP1-like functions in legumes. Plant Physiol 142: 972-983.
Berbel A, Navarro C, Ferrándiz C, Cañas LA, Madueño F, Beltrán JP (2001). Analysis of PEAM4, the pea AP1 functional homologue, supports a model for AP1‐like genes controlling both floral meristem and floral organ identity in different plant species. Plant J 25: 441-451.
Bowman JL, Alvarez J, Weigel D, Meyerowitz EM, Smyth DR (1993). Control of flower development in Arabidopsis thaliana by APETALA1 and interacting genes. Development 119: 721-743.
Carpenter R, Coen ES (1990). Floral homeotic mutations produced by transposon-mutagenesis in Antirrhinum majus. Gene Dev 4: 1483-1493.
Chi Y, Huang F, Liu H, Yang S, Yu D (2011). An APETALA1-like gene of soybean regulates flowering time and specifies floral organs. J Plant Physiol 168: 2251-2259.
Coen ES, Romero J, Doyle S, Elliott R, Murphy G, Carpenter R (1990). floricaula: a homeotic gene required for flower development in Antirrhinum majus. Cell 63: 1311-1322.
Davis PH (1965). Flora of Turkey and the East Aegean Islands (Vol. 1). Edinburgh, UK: Edinburgh University Press, pp. 95-206.
Doyle JJ, Doyle JL (1990). Isolation of plant DNA from fresh tissue. Focus 12: 13-15. Ferrándiz C, Navarro C, Gomez MD, Canas LA, Beltran JP (1999). Flower development in Pisum sativum: from the war of the whorls to the battle of the common primordia. Dev Genet 25: 280-290.
Ge J, Zhao D, Han C, Wang J, Hao Z, Tao J (2014). Cloning and expression of floral organ development-related genes in herbaceous peony (Paeonia lactiflora Pall.). Mol Biol Rep 41: 6493-6503.
Gil-Humanes J, Pistón F, Martín A, Barro F (2009). Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids. BMC Plant Biol 9: 66. Hamès C, Ptchelkine D, Grimm C, Thevenon E, Moyroud E, Gérard F, Martiel JL, Benlloch R, Parcy F, Müller CW (2008). Structural basis for LEAFY floral switch function and similarity with helix turn-helix proteins. E