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Dergiler / Turkish Journal of Biology / 2016 / Cilt: 40 - Sayı: 6

Cloning and abiotic stress resistance analyses of a new proline-glycine-alanine histidine-rich protein gene from Ipomoea batatas (L.) Lam.

Bin YOUNG
Huan-Huan SHAO
Xin-Rong MA
Xiang TAO
Sayfa
1148–1157
Yayın Tarihi
2022-10-17
DOI
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

This study revealed that IbPGAHRP1 is a flower-specific gene and should play an important role in response to abiotic stresses.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Sweet potato [Ipomoea batatas (L.) Lam.] is one of the top six most important crops in the world in terms of production.
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Temel Bulgular & Veri:It has the capacity to tolerate marginal growing conditions better than most other crops.
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Sonuç & Katkı:This study revealed that IbPGAHRP1 is a flower-specific gene and should play an important role in response to abiotic stresses.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

Sweet potato [Ipomoea batatas (L.) Lam.] is one of the top six most important crops in the world in terms of production. It has the capacity to tolerate marginal growing conditions better than most other crops. In this study, a proline- and glycine-rich protein encoding gene, IbPGAHRP1, was isolated and characterized from the transcriptome of sweet potato cultivar Xushu 18. Sequence analyses showed that proline, glycine, alanine, and histidine account for 62.6% of the total amino acid residues. Two proline-rich tandem repeats, an alanine-rich repeat, a histidine-rich tandem repeat, and five XYPP motifs were present in IbPGAHRP1. Sequence similarity analysis showed that IbPGAHRP1 shared 41% similarity with GmGPRP4, indicating that IbPGAHRP1 should be a GPRP-like protein and play an important role in stress resistance. RT-PCR results showed that IbPGAHRP1 was specifically expressed in the sweet potato flower. In vivo expression of IbPGAHRP1 demonstrated that it enhanced the cold and drought resistance in the yeast recombinant, but could not trigger the same changes in Escherichia coli. This study revealed that IbPGAHRP1 is a flower-specific gene and should play an important role in response to abiotic stresses.

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Bu Makaleye Atıf Yap

YOUNG, B., SHAO, H., MA, X., TAO, X. (2022). Cloning and abiotic stress resistance analyses of a new proline-glycine-alanine histidine-rich protein gene from Ipomoea batatas (L.) Lam.. Turkish Journal of Biology, Cilt: 40 - Sayı: 6, 1148–1157. https://ajindex.com/articles/600444

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Kaynakça (15)

  1. Ashraf M, Foolad M (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59: 206-216.
  2. Baneyx F, Mujacic M (2004). Recombinant protein folding and misfolding in Escherichia coli. Nat Biotechnol 22: 1399-1408.
  3. Blanvillain R, Wei S, Wei P, Kim JH, Ow DW (2011). Stress tolerance to stress escape in plants: role of the OXS2 zinc-finger transcription factor family. EMBO J 30: 3812-3822.
  4. Cassab GI (1998). Plant cell wall proteins. Annu Rev Plant Biol 49: 281-309.
  5. Chen J, Zhao J, Ning J, Liu Y, Xu J, Tian S, Zhang L, Sun MX (2014). NtProRP1, a novel proline-rich protein, is an osmotic stress-responsive factor and specifically functions in pollen tube growth and early embryogenesis in Nicotiana tabacum. Plant Cell Environ 37: 499-511.
  6. Dinneny JR, Long TA, Wang JY, Jung JW, Mace D, Pointer S, Barron C, Brady SM, Schiefelbein J, Benfey PN (2008). Cell identity mediates the response of Arabidopsis roots to abiotic stress. Science 320: 942-945.
  7. Franks SJ (2011). Plasticity and evolution in drought avoidance and escape in the annual plant Brassica rapa. New Phytol 190: 249-257.
  8. Gietz RD, Schiestl RH (2007). High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2: 31-34.
  9. Gothandam KM, Nalini E, Karthikeyan S, Shin JS (2010). OsPRP3, a flower specific proline-rich protein of rice, determines extracellular matrix structure of floral organs and its overexpression confers cold-tolerance. Plant Mol Biol 72: 125-135.
  10. Hare PD, Cress WA, Van Staden J (1998). Dissecting the roles of osmolyte accumulation during stress. Plant Cell Environ 21:535-553.
  11. Hasanuzzaman M, Nahar K, Alam MM, Roychowdhury R, Fujita M (2013). Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. Int J Mol Sci 14: 9643-9684.
  12. He Y, Amasino RM (2005). Role of chromatin modification in flowering-time control. Trends Plant Sci 10: 30-35.
  13. Hong ZL, Lakkineni K, Zhang ZM, Verma DPS (2000). Removal of feedback inhibition of Δ(1)-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress. Plant Physiol 122: 1129-1136.
  14. Hopkins W, Hüner N (2009). Acclimation to environmental stress. In: Hopkins WG, Hüner NPA, editors. Introduction to Plant Physiology. Hoboken, NJ, USA: Wiley, pp. 241-260.
  15. Janska A, Marsik P, Zelenkova S, Ovesna J (2010). Cold stress and acclimation-what is important for metabolic adjustment? Plant Biology 12: 395-405.