KaydedilenlerDerginizi ekleyin

Dergiler / Turkish Journal of Biology / 2016 / Cilt: 40 - Sayı: 4

Pluronic PF68 increases transfection efficiency in electroporationof mesenchymal stem cells

Ferruh ÖZCAN
Mehmet Emir YALVAÇ
Safa AYDIN
Fikrettin ŞAHİN
Sayfa
747–754
Yayın Tarihi
2022-10-17
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The study suggested that PF68 might be a safe and reliable chemical to use for increasing transfection efficiency during electroporation and chemical transfections.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Electroporation is a safe and efficient method to transfect human tooth germ stem cells (hTGSCs), which are mainly mesenchymal stem cells (MSCs).
📊
Temel Bulgular & Veri:In this study, pluronic F68 (PF68), a nonionic and low-foaming surfactant, was used for the first time to increase the transfection efficiency in electroporation of hTGSCs.
💡
Sonuç & Katkı:The study suggested that PF68 might be a safe and reliable chemical to use for increasing transfection efficiency during electroporation and chemical transfections.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

Electroporation is a safe and efficient method to transfect human tooth germ stem cells (hTGSCs), which are mainly mesenchymal stem cells (MSCs). In this study, pluronic F68 (PF68), a nonionic and low-foaming surfactant, was used for the first time to increase the transfection efficiency in electroporation of hTGSCs. Our results revealed that when PF68 was used in the preincubation, electroporation, and recovery medium, it dramatically increased transfection efficiency. Moreover, use of PF68 did not alter the differentiation capacity of hTGSCs, whereas cells electroporated without PF68 had significantly lower expression of Oct-4, pluripotency marker gene, and alkaline phosphatase (ALP) activity upon osteogenic differentiation. We found that PF68 increases transfection efficiency in both electroporation and chemical transfection of cancer cells lines MCF-7 and HeLa. The study suggested that PF68 might be a safe and reliable chemical to use for increasing transfection efficiency during electroporation and chemical transfections.

🔖

Bu Makaleye Atıf Yap

ÖZCAN, F., YALVAÇ, M. E., AYDIN, S., ŞAHİN, F. (2022). Pluronic PF68 increases transfection efficiency in electroporationof mesenchymal stem cells. Turkish Journal of Biology, Cilt: 40 - Sayı: 4, 747–754. https://ajindex.com/articles/600440

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (11)

  1. ReferencesAyuni EL, Gazdhar A, Giraud MN, Kadner A, Gugger M, Cecchini M, Caus T, Carrel TP, Schmid RA, Tevaearai HT (2010). In vivo electroporation mediated gene delivery to the beating heart. PLoS One 5: e14467. Campagnoli C, Roberts IA, Kumar S, Bennett PR, Bellantuono I, Fisk NM (2001). Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone mar-row. Blood 98: 2396-2402.
  2. Collins JM, Despa F, Lee RC (2007). Structural and functional recov-ery of electropermeabilized skeletal muscle in-vivo after treat-ment with surfactant poloxamer 188. Biochim Biophys Acta 1768: 1238-1246.
  3. Dogan A, Yalvac ME, Sahin F, Kabanov AV, Palotas A, Rizvanov AA (2012). Differentiation of human stem cells is promoted by amphiphilic pluronic block copolymers. Int J Nanomedicine 7: 4849-4860.
  4. Dogan A, Yalvac ME, Yilmaz A, Rizvanov A, Sahin F (2013). Effect of F68 on cryopreservation of mesenchymal stem cells derived from human tooth germ. Appl Biochem Biotechnol 171: 1819-1831.
  5. Ferreira E, Potier E, Logeart-Avramoglou D, Salomskaite-Davalgiene S, Mir LM, Petite H (2008). Optimization of a gene electro-transfer method for mesenchymal stem cell transfection. Gene Ther 15: 537-544.
  6. Gehl J (2003). Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research. Acta Physiol Scand 177: 437-447. Graziano A, d’Aquino R, Laino G, Papaccio G (2008). Dental pulp stem cells: a promising tool for bone regeneration. Stem Cell Rev 4: 21-26.
  7. Hartikka J, Sukhu L, Buchner C, Hazard D, Bozoukova V, Margalith M, Nishioka WK, Wheeler CJ, Manthorp M, Sawdey M (2001). Electroporation-facilitated delivery of plasmid DNA in skeletal muscle: plasmid dependence of muscle damage and effect of poloxamer 188. Mol Ther 4: 407-415.
  8. Ikeda E, Yagi K, Kojima M, Yagyuu T, Ohshima A, Sobajima S, Tado-koro M, Katsube Y, Isoda K, Kondoh M et al. (2008). Multipo-tent cells from the human third molar: feasibility of cell-based therapy for liver disease. Differentiation 76: 495-505.
  9. Peister A, Mellad JA, Wang M, Tucker HA, Prockop DJ (2004). Stable transfection of MSCs by electroporation. Gene Ther 11: 224-228.
  10. Sharma V, Stebe K, Murphy JC, Tung L (1996). Poloxamer 188 de-creases susceptibility of artificial lipid membranes to electro-poration. Biophys J 71: 3229-3241.
  11. Tasli PN, Yalvac ME, Sofiev N, Sahin F (2013). Effect of F68, F127, and P85 pluronic block copolymers on odontogenic differen-tiation of human tooth germ stem cells. J Endod 3