Dergiler / Turkish Journal of Biology / 2016 / Cilt: 40 - Sayı: 3

Comparative and phylogenetic analysis of zinc transporter genes/proteins in plants

Sayfa
600–611
Yayın Tarihi
2022-10-17
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The findings of this study will be valuable theoretical knowledge for feature studies in terms of understanding the gene and protein features of Zn transporters in various plants.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Zinc is an important catalytic element for more than 300 enzymes and plays a structural role in the stabilization of many proteins.
📊
Temel Bulgular & Veri:Protein domain analysis showed that identified Zn transporter proteins belong to the ZIP protein family (PF02535).
💡
Sonuç & Katkı:The findings of this study will be valuable theoretical knowledge for feature studies in terms of understanding the gene and protein features of Zn transporters in various plants.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

Zinc is an important catalytic element for more than 300 enzymes and plays a structural role in the stabilization of many proteins. Protein domain analysis showed that identified Zn transporter proteins belong to the ZIP protein family (PF02535). Zn transporter sequences were found to have similar molecular weights (33.1-51.4 kDa) and amino acid lengths (306-478 amino acids) with 5.31-8.92 pI values. Subcellular localization of Zn transporters was predicted as the plasma membrane. They had 6-9 putative transmembrane domains with a variable region between TM-3 and TM-4, which could contain a potential histidine-rich metal-binding domain. Moreover, alignment analysis showed that the TM-2, -4, and -5 domains could be potential metal-binding sites because they contain highly conserved His residues. Based on a homology search, the retrieved sequences were identified as corresponding homologs of either Arabidopsis thaliana or Oryza sativa. Phylogenetic analysis also supported that A. thaliana and O. sativa sequences could be used as a reference/benchmark to identify Zn transporter homologs in various plant species. The findings of this study will be valuable theoretical knowledge for feature studies in terms of understanding the gene and protein features of Zn transporters in various plants.

🔖

Bu Makaleye Atıf Yap

FİLİZ, E., VATANSEVER, R., ÖZTİĞİT, İ. İ. (2022). Comparative and phylogenetic analysis of zinc transporter genes/proteins in plants. Turkish Journal of Biology, Cilt: 40 - Sayı: 3, 600–611. https://ajindex.com/articles/600406

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (12)

  1. Assunção AG, Herrero E, Lin YF, Huettel B, Talukdar S, Smaczniak C, Immink RH, Eldik Mv, Fiers M, Schat H et al. (2010). Arabidopsis thaliana transcription factors bZIP19 and bZIP23 regulate the adaptation to zinc deficiency. P Natl Acad Sci USA 107: 10296-10301.
  2. Connolly EL, Fett JP, Guerinot ML (2002). Expression of the IRT1 metal transporter is controlled by metals at the levels of transcript and protein accumulation. Plant Cell 14: 1347-1357.
  3. Eng BH, Guerinot ML, Eide D, Saier MH Jr (1998). Sequence analyses and phylogenetic characterization of the ZIP family of metal ion transport proteins. J Membrane Biol 166: 1-7.
  4. Fox TC, Guerinot ML (1998). Molecular biology of cation transport in plants. Annu Rev Plant Biol 49: 669-696.
  5. Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD, Bairoch A (2005). Protein identification and analysis tools on the ExPASy server. In: Walker JM, editor. The Proteomics Protocols Handbook. New York, NY, USA: Humana Press, pp. 571-607.
  6. Goodstein DM, Shu S, Howson R, Neupane R, Hayes RD, Fazo J, Mitros T, Dirks W, Hellsten U, Putnam N (2012). Phytozome: a comparative platform for green plant genomics. Nucleic Acids Res 40: 1178-1186.
  7. Grotz N, Fox T, Connolly E, Park W, Guerinot ML, Eide D (1998). Identification of a family of zinc transporter genes from Arabidopsis that respond to zinc deficiency. P Natl Acad Sci USA 95: 7220-7224.
  8. Guerinot ML (2000). The ZIP family of metal transporters. BBABiomembranes 1465: 190-198.
  9. Guo AY, Zhu QH, Chen X, Luo JC (2007). GSDS: a gene structure display server. Yi Chuan 29: 1023-1026 (in Chinese with abstract in English). Ishimaru Y, Suzuki M, Kobayashi T, Takahashi M, Nakanishi H, Mori S, Nishizawa NK (2005). OsZIP4, a novel zinc-regulated zinc transporter in rice. J Exp Bot 56: 3207-3214.
  10. Krogh A, Larsson B, von Heijne G, Sonnhammer ELL (2001). Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 305: 567-580.
  11. Li S, Zhou X, Huang Y, Zhu L, Zhang S, Zhao Y, Chen R (2013). Identification and characterization of the zinc-regulated transporters, iron-regulated transporter-like protein (ZIP) gene family in maize. BMC Plant Biol 13: 114. Lin YF, Liang HM, Yang SY, Boch A, Clemens S, Chen CC, Yeh KC (2009). Arabidopsis IRT3 is a zinc‐regulated and plasma membrane localized zinc/iron transporter. New Phytol 182: 392-404.
  12. Lopez-Millan AF, Ellis DR, Grusak MA (2004). Identification and characterization of several new members of